Key Laboratory of Agricultural Biotechnology of Liaoning Province, College of Biosciences and Biotechnology, Shenyang Agriculture University, Shenyang, China.
PeerJ. 2024 Jun 18;12:e17537. doi: 10.7717/peerj.17537. eCollection 2024.
The domain of unknown function 668 (DUF668) is a gene family that may play a key role in plant growth and development as well as in responding to adversity coercion stresses. However, the DUF668 gene family has not yet been well identified and characterized in tomato. In this study, a total of nine putative genes were identified in tomato, distributed on six chromosomes. Phylogenetic analyses revealed that SlDUF668 proteins were classified into two major groups. Members within the same group largely displayed analogous gene structure and conserved motif compositions. Several -elements were exhibited in the upstream sequences of the genes, including elements implicated in plant growth and development processes, abiotic stress and hormone responses. Further, the study assessed the expression patterns of the SlDUF668 gene family in various tomato tissues, five plant hormones treatments, three abiotic stresses using qRT-PCR. The genes expressed ubiquitously in various tissues, and five genes (, , , and ) showed tissue specificity. And genes responded to abiotic stresses such as salt, drought and cold to varying degrees. Overall, our study provided a base for the tomato DUF668 gene family and laid a foundation for further understanding the functional characteristics of genes in tomato plants.
未知功能域 668（DUF668）基因家族是一类可能在植物生长发育以及逆境胁迫响应中发挥关键作用的基因家族。然而，番茄中的 DUF668 基因家族尚未得到很好的鉴定和特征描述。在本研究中，在番茄中鉴定到了总共 9 个假定的 SlDUF668 基因，分布在 6 条染色体上。系统进化分析表明，SlDUF668 蛋白被分为两个主要组。同一组内的成员具有相似的基因结构和保守的基序组成。在 SlDUF668 基因的上游序列中存在几个顺式作用元件，包括与植物生长发育过程、非生物胁迫和激素响应相关的元件。此外，本研究还通过 qRT-PCR 检测了 SlDUF668 基因家族在番茄不同组织、五种植物激素处理、三种非生物胁迫下的表达模式。结果表明，SlDUF668 基因在各种组织中广泛表达，其中 5 个基因（、、、和）具有组织特异性。并且，一些基因对盐、干旱和低温等非生物胁迫有不同程度的响应。总的来说，本研究为番茄 DUF668 基因家族提供了一个基础，为进一步了解番茄植物中 SlDUF668 基因的功能特征奠定了基础。
