Kitagawa S, Obata T, Willingham M C, Cheng S Y
Endocrinology. 1987 Jun;120(6):2591-6. doi: 10.1210/endo-120-6-2591.
The effects of thyroid hormones on morphology and growth were studied in rat pituitary tumor GH3 cells using medium containing serum depleted of thyroid hormones. T3 and T4 induce the cells to change from a flattened fibroblastic morphology to a rounded or spindle-shaped morphology. The induction in morphological changes is T3 and T4 specific and dose dependent. Thyronine and rT3 are ineffective in inducing morphological changes; the half-maximal effective concentrations for T3 and T4 are 0.3 and 2 nM, respectively. Concomitantly, T3 stimulates cell growth, as indicated by a 2-fold reduction in doubling time and a 2-fold increase in mitotic rate. The growth-stimulating effect has the same analog specificity and dose dependency as the morphological changes. The morphological changes could be potentially useful for evaluating the biological effects of T3 and its analogs and in studying the mechanism of thyroid hormone action.
使用不含甲状腺激素的血清培养基，在大鼠垂体瘤GH3细胞中研究了甲状腺激素对形态和生长的影响。T3和T4诱导细胞从扁平的成纤维细胞形态转变为圆形或纺锤形形态。形态变化的诱导具有T3和T4特异性且呈剂量依赖性。甲状腺素和反式T3在诱导形态变化方面无效；T3和T4的半数最大有效浓度分别为0.3和2 nM。同时，T3刺激细胞生长，表现为倍增时间减少2倍和有丝分裂率增加2倍。生长刺激作用与形态变化具有相同的类似物特异性和剂量依赖性。形态变化可能有助于评估T3及其类似物的生物学效应以及研究甲状腺激素作用机制。
