[The assistant effects of porcine fibrin sealant in improving stone clearance rate in flexible ureteroscopy lithotripsy in porcine kidney model].

To investigate the feasibility of using a porcine fibrin sealant to wrap and remove kidney calculi fragments through an isolated porcine kidney model. In the isolated porcine kidney stone model (implanted with 100 mg, air dried, ≤1 mm human stone fragments, =6;implanted with 100 mg, air dried, ≤3 mm human stone fragments, =6), the ureteral soft mirror combined with the 12/14Fr UAS was used to test the effect of stone extraction using only two stone extraction methods: basket extraction (control group, ≤1 mm stone fragments, =3; ≤3 mm stone fragments, =3) and basket-sealant extraction (test group, ≤1 mm stone fragments, =3; ≤3 mm stone fragments, =3). Compare the stone removal rate and operation time of the two stone retrieval methods. The sealant was put into urine of normal human and observed. Porcine Fibrin Sealant can form a gel in saline and urine and adhere and wrap stone fragments. The time of procedures of test (basket-sealant) and control (basket) group in kidneys implanted with ≤ 1 mm stone fragments were (14.0±4.2) and (29.0±0.7)min (<0.05) stone clearance rates were (90.9±1.4)% and (48.4±15.7)% (<0.05), respectively. In kidneys implanted with ≤ 3 mm fragments, time of procedures were (12.8±4.0) and (30.0±0)min (<0.05) Stone clearance rates were (91.1±5.0)% and (20.7±8.0)% (<0.05). The Sealant dissolves by itself in normal human urine and normal saline at 37 ℃ for 24 hours. The appropriate concentration of Porcine Fibrin Sealant assisted stone retrieval may become a new method for removing small stone fragments in retrograde intrarenal surgery.