Wei Dawei, Raza Sayed Haidar Abbas, Wang Xingping, Khan Rajwali, Lei Zhaoxiong, Zhang Guijie, Zhang Jiupan, Luoreng Zhuoma, Ma Yun, Alamoudi Muna O, Aloufi Bandar Hamad, Alshammari Ahmed Mohajja, Abd El-Aziz Ayman Hassan, Alhomrani Majid, Alamri Abdulhakeem S
School of Agriculture, Ningxia University, Yinchuan, China.
Key Laboratory of Ruminant Molecular Cell Breeding, Ningxia Hui Autonomous Region, Yinchuan, China.
Front Vet Sci. 2022 May 26;9:853819. doi: 10.3389/fvets.2022.853819. eCollection 2022.
As a member of the large tumor suppressor () gene family, 1 plays an important role in regulating muscle growth and development. In this study, we determined the distinct exhibit patterns of tissue expression of bovine 1. Further, we determined the functional proximal minimal promoter of bovine 1 and identified the key transcription factors in the core promoter region to elucidate its molecular regulation mechanism. The results showed that bovine 1 was highly expressed in the and upregulation in infancy muscle. An electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation (ChIP) assay in combination with site-directed mutation and small interfering RNA (siRNA) interference demonstrated that myogenic differentiation 1 (Myod1) and myocyte enhancer factor 2A (MEF2A) binding in the core promoter region (-298/-123 bp) play important roles in the transcriptional regulation of the bovine 1 promoter. Taken together, these interactions provide insight into the regulatory mechanisms of 1 transcription in mediating skeletal muscle growth in cattle.
作为大肿瘤抑制因子（）基因家族的一员，1在调节肌肉生长和发育中发挥着重要作用。在本研究中，我们确定了牛1组织表达的独特表现模式。此外，我们确定了牛1的功能性近端最小启动子，并鉴定了核心启动子区域中的关键转录因子，以阐明其分子调控机制。结果表明，牛1在 中高表达，在幼年肌肉中上调。电泳迁移率变动分析（EMSA）和染色质免疫沉淀（ChIP）分析结合定点突变和小干扰RNA（siRNA）干扰表明，核心启动子区域（-298/-123 bp）中的生肌分化1（Myod1）和肌细胞增强因子2A（MEF2A）结合在牛1启动子的转录调控中起重要作用。综上所述，这些相互作用为1转录在介导牛骨骼肌生长中的调控机制提供了见解。
