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中国互助白牦牛的线粒体基因组特征与系统发育研究

Mitogenome characterization and phylogeny of Huzhu white yak () in China.

作者信息

Luo Jing, Li Guangzhen, Li Ruizhe, Yang Yongqing, He Decang, Liu Wenxian, Yosri Mohammed, Ma Zhijie

机构信息

Qinghai Academy of Animal Science and Veterinary Medicine, Qinghai University, Xining, China.

Plateau Livestock Genetic Resources Protection and Innovative Utilization Key Laboratory of Qinghai Province, Xining, China.

出版信息

Mitochondrial DNA B Resour. 2022 Jun 7;7(6):901-904. doi: 10.1080/23802359.2022.2073835. eCollection 2022.

Abstract

White yak is a unique and precious economic livestock animal in the world. In this study, the mitogenome of Huzhu white yak was firstly sequenced using Illumina high-throughput sequencing technique and then the assembly was annotated. We also explored mitogenome characterization and phylogeny of Huzhu white yak. Our results showed that the mitogenome of Huzhu white yak is a circular molecule with 16,323bp length including a non-coding control region (D-loop), two ribosomal RNA genes ( and ), 22 transfer RNA genes and 13 protein-coding genes. The contents of four nucleotides (A, G, C and T) were 33.71%, 13.21%, 25.80%, and 27.28%, respectively, yielding a lower GC content (39.01%) than AT (60.99%). Phylogenetic analysis suggested that Huzhu white yak possessed the closest relationships with Huanhu, Jiulong, Datong, Jinchuan, Sibu, Ashdan and Pali yak breeds, and closer to wild yak and Bazhou breed.

摘要

白牦牛是世界上独特而珍贵的经济家畜。本研究首先利用Illumina高通量测序技术对白牦牛的线粒体基因组进行测序,然后进行组装注释。我们还对白牦牛的线粒体基因组特征和系统发育进行了探索。结果表明,白牦牛的线粒体基因组是一个长度为16323bp的环状分子,包括一个非编码控制区(D-loop)、两个核糖体RNA基因(和)、22个转运RNA基因和13个蛋白质编码基因。四种核苷酸(A、G、C和T)的含量分别为33.71%、13.21%、25.80%和27.28%,GC含量(39.01%)低于AT含量(60.99%)。系统发育分析表明,白牦牛与环湖、九龙、大通、金川、斯布、阿什旦和帕里牦牛品种关系最近,与野牦牛和巴州品种关系较近。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4946/9176365/5502af1b5d00/TMDN_A_2073835_F0001_C.jpg

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