Liu Yan, Shao Yang, Wang Lu, Lu Weilai, Li Shihua, Xu Diandou, Fu Yu Vincent
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
Savaid Medical School, University of Chinese Academy of Sciences, Beijing 100049, China.
Environ Technol Innov. 2022 Aug;27:102715. doi: 10.1016/j.eti.2022.102715. Epub 2022 Jun 6.
The many instances of COVID-19 outbreaks suggest that cold chains are a possible route for the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, owing to the low temperatures of cold chains, which are normally below 0 °C, there are limited options for virus inactivation. Here, high-energy electron beam (E-beam) irradiation was used to inactivate porcine epidemic diarrhea virus (PEDV) under simulated cold chain conditions. This coronavirus was used as a surrogate for SARS-CoV-2. The possible mechanism by which high-energy E-beam irradiation inactivates PEDV was also explored. An irradiation dose of 10 kGy reduced the PEDV infectious viral titer by 1.68-1.76 logTCID L in the cold chain environment, suggesting that greater than 98.1% of PEDV was inactivated. E-beam irradiation at 5-30 kGy damaged the viral genomic RNA with an efficiency of 46.25%-92.11%. The integrity of the viral capsid was disrupted at 20 kGy. The rapid and effective inactivation of PEDV at temperatures below freezing indicates high-energy E-beam irradiation as a promising technology for disinfecting SARS-CoV-2 in cold chain logistics to limit the transmission of COVID-19.
新型冠状病毒肺炎（COVID-19）疫情的诸多实例表明，冷链是严重急性呼吸综合征冠状病毒2（SARS-CoV-2）传播的一条可能途径。然而，由于冷链温度通常低于0°C，病毒灭活的选择有限。在此，利用高能电子束（E-beam）辐照在模拟冷链条件下灭活猪流行性腹泻病毒（PEDV）。该冠状病毒被用作SARS-CoV-2的替代物。还探讨了高能E-beam辐照灭活PEDV的可能机制。在冷链环境中，10 kGy的辐照剂量使PEDV感染性病毒滴度降低了1.68-1.76 logTCID₅₀/mL，这表明超过98.1%的PEDV被灭活。5-30 kGy的E-beam辐照对病毒基因组RNA的损伤效率为46.25%-92.11%。在20 kGy时病毒衣壳的完整性被破坏。在冷冻温度下PEDV的快速有效灭活表明，高能E-beam辐照是一种很有前景的技术，可用于冷链物流中对SARS-CoV-2进行消毒，以限制COVID-19的传播。
