Genomic insights into and .

BACKGROUND

Antimicrobial and antifungal species are essential members of the healthy human microbiota. Several different species of lactobacilli that naturally inhabit the human body have been explored for their probiotic capabilities including strains of the species . However, (identified by 16S rRNA gene sequencing) has been associated with urogenital symptoms. Recently a new sister taxon of was described: . is also posited to have probiotic qualities.

METHODS

Here, we present a genomic investigation of all ( = 79) publicly available genome assemblies for both species. These strains include isolates from the vaginal tract, gastrointestinal tract, urinary tract, oral cavity, wounds, and lungs.

RESULTS

The two species cannot be distinguished from short-read sequencing of the 16S rRNA as the full-length gene sequences differ only by two nucleotides. Based upon average nucleotide identity (ANI), we identified 20 strains deposited as that are in fact representatives of . Investigation of the genic content of the strains of these two species suggests recent divergence and/or frequent gene exchange between the two species. The genomes frequently harbored intact prophage sequences, including prophages identified in strains of both species. To further explore the antimicrobial potential associated with both species, genome assemblies were examined for biosynthetic gene clusters. Gassericin T and S were identified in 46 of the genome assemblies, with all strains including one or both bacteriocins. This suggests that the properties once ascribed to may better represent the species.