Stafanger G, Bisgaard H, Pedersen M, Mørkassel E, Koch C
Eur J Respir Dis. 1987 Mar;70(3):157-62.
N-acetylcysteine (NAC) is widely used as a mucolytic agent, but the clinical and pharmacological effects of NAC are still unclear. It has recently been claimed in animal studies that NAC will stimulate ciliary beating frequency at low concentrations, while inhibiting beating at higher concentrations. Using a microphoto-oscillographic method combined with microperfusion technique, we studied the direct effect of NAC on human nasal cilia. NAC caused a direct dose- and time-related decrease in ciliary beating frequency, which was detectable at 2 mg/ml and reached statistically significant levels at 20 mg/ml. NAC at 200 mg/ml caused total cessation of movements within 15 s but this effect was completely reversible within 15 min of perfusion with medium alone. At no concentrations of NAC was beating frequency increased over base-line. NAC, however, had no effect on beating pattern. NAC seems to have a selective and fully reversible inhibitory effect on the beating frequency of human cilia in vitro.
N-乙酰半胱氨酸（NAC）作为一种黏液溶解剂被广泛使用，但其临床和药理作用仍不明确。最近在动物研究中声称，NAC在低浓度时会刺激纤毛摆动频率，而在高浓度时则抑制摆动。我们采用显微光振荡描记法结合微量灌注技术，研究了NAC对人鼻纤毛的直接作用。NAC导致纤毛摆动频率出现直接的剂量和时间依赖性降低，在2mg/ml时即可检测到，在20mg/ml时达到统计学显著水平。200mg/ml的NAC在15秒内导致运动完全停止，但在用单纯培养基灌注15分钟内这种作用完全可逆。在任何浓度的NAC下，摆动频率均未超过基线水平而增加。然而，NAC对摆动模式没有影响。NAC似乎在体外对人纤毛的摆动频率具有选择性且完全可逆的抑制作用。
