Sun Jun, Li Gui-Sheng
Laboratory of Plant Resource Conservation and Utilization, Jishou University, Jishou 416000, China.
AoB Plants. 2022 May 5;14(3):plac018. doi: 10.1093/aobpla/plac018. eCollection 2022 Jun.
Lycophytes are the earliest vascular plants and is the most studied genus among them. Prostrate shoots are produced during early growth and erect shoots emerge later in . , thus providing an opportunity for exploring the evolution of the mechanism underlying the transition between growth phases. Six libraries were sequenced for the prostrate and the erect shoots, and a total of 206 768 genes were identified. Some genes were differentially expressed in prostate and erect shoot, with relatively high expression in the prostate shoots being related to hormone responses and defence reactions, while higher expression in the erect shoots was related to spore formation and shoot development. Some genes possessed a miR156 binding site and were highly expressed in the erect shoots, while -like genes were more highly expressed in the prostrate shoots but simultaneously lacked any miR172 binding site. MiR156 was detected at a higher concentration in the prostrate shoots. Thus, the mechanism for the vegetative to reproductive transition of sporophytes probably originated in the common ancestor of vascular plants and must have experienced stepwise development during evolution.
石松类植物是最早的维管植物，并且是其中研究最多的属。早期生长阶段产生平卧茎，直立茎稍后出现，从而为探索生长阶段转变背后机制的进化提供了机会。对平卧茎和直立茎进行了六个文库的测序，共鉴定出206768个基因。一些基因在平卧茎和直立茎中差异表达，平卧茎中相对较高的表达与激素反应和防御反应有关，而直立茎中较高的表达与孢子形成和茎发育有关。一些基因具有miR156结合位点且在直立茎中高表达，而类似AP2的基因在平卧茎中表达更高但同时缺乏任何miR172结合位点。在平卧茎中检测到更高浓度的miR156。因此，孢子体从营养生长到生殖生长转变的机制可能起源于维管植物的共同祖先，并且在进化过程中必定经历了逐步发展。
