用亚胺硫醇盐对预制脂质体进行硫醇化修饰。

Thiolation of preformed liposomes with iminothiolane.

作者信息

Lasch J, Niedermann G, Bogdanov A A, Torchilin V P

出版信息

FEBS Lett. 1987 Apr 6;214(1):13-6. doi: 10.1016/0014-5793(87)80004-2.

Abstract

Preformed phosphatidylethanolamine-containing liposomes were thiolated with 2-iminothiolane (Traut's reagent) and subsequently activated by mixed disulfide formation with 5,5'-dithiobis(2-nitrobenzoic acid). Up to 65% of amino groups of the outer liposomal lamella, corresponding to 230 SH-groups per vesicle, were modified. Covalent attachment of thiolated alpha-chymotrypsin to these thiol-liposomes via S-S bridges yielded a protein/lipid ratio of 3.6 X 10(-4) mol enzyme/mol lipid.

摘要

预先形成的含磷脂酰乙醇胺脂质体用2-亚氨基噻烷（特劳特试剂）进行硫醇化，随后通过与5,5'-二硫代双（2-硝基苯甲酸）形成混合二硫键进行活化。外脂质体片层高达65%的氨基被修饰，相当于每个囊泡有230个巯基。通过S-S桥将硫醇化的α-胰凝乳蛋白酶共价连接到这些硫醇脂质体上，得到的蛋白质/脂质比为3.6×10⁻⁴摩尔酶/摩尔脂质。