通过IRAP和REMAP标记揭示的来自伊朗北部的节节麦种质的高遗传多样性。

High genetic diversity in Aegilops tauschii Coss. accessions from North Iran as revealed by IRAP and REMAP markers.

作者信息

Minaei Sona, Mohammadi Seyyed Abolghasem, Sabouri Atefeh, Dadras Ahmad Reza

机构信息

Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.

Center of Excellence in Cereal Molecular Breeding, University of Tabriz, Tabriz, Iran.

出版信息

J Genet Eng Biotechnol. 2022 Jun 13;20(1):86. doi: 10.1186/s43141-022-00363-y.

DOI:10.1186/s43141-022-00363-y

PMID:35696009

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9192835/

Abstract

BACKGROUND

Aegilops tauschii Coss. as a donor of wheat D genome has an important role in wheat breeding programs. Genetic and phylogeographic diversity of 79 Ae. tauschii accessions collected from north and northwest of Iran were analyzed based on retroelement insertional polymorphisms using inter-retrotransposon amplified polymorphism (IRAP) and retrotransposon-microsatellite amplified polymorphism (REMAP) markers.

RESULTS

In total, 306 and 151 polymorphic bands were amplified in IRAP and REMAP analyses, respectively. As a result, a high level of polymorphism was observed among the studied accessions as revealed by an average of 25.5 bands per primer/primer combination and mean PIC value of 0.47 in IRAP and an average of 25.16 bands per primer combination and mean PIC value of 0.47 in REMAP. Genetic relationships of the accessions were analyzed using distance- and model-based cluster analyses.

CONCLUSION

The result showed that genetic distance did not seem to be related to geographic distribution, and the accessions could be divided into three groups, which was further supported by principal coordinate analysis. These results on genetic diversity and population structure of Ae. tauschii in Iran should provide important knowledge on genetic resources and their applications in wheat breeding programs.

摘要

背景

节节麦作为小麦D基因组的供体，在小麦育种计划中具有重要作用。利用反转录转座子间扩增多态性（IRAP）和反转录转座子-微卫星扩增多态性（REMAP）标记，基于反转录元件插入多态性，对从伊朗北部和西北部收集的79份节节麦材料的遗传和系统地理多样性进行了分析。

结果

在IRAP和REMAP分析中，分别共扩增出306条和151条多态性条带。结果显示，在所研究的材料中观察到高水平的多态性，IRAP分析中每个引物/引物组合平均有25.5条带，平均PIC值为0.47；REMAP分析中每个引物组合平均有25.16条带，平均PIC值为0.47。利用基于距离和模型的聚类分析方法对材料的遗传关系进行了分析。