Stich H F, Wei L, Lam P
Cancer Lett. 1978 Oct;5(4):199-204. doi: 10.1016/s0304-3835(78)80039-1.
Reducing agents and cysteine, cysteamine, glutathione, ascorbic acid and H2O2 with and without the addition of Cu2+ did not increase significantly the frequency of mutations in the Salmonella test at non-toxic concentrations but triggered a marked DNA repair synthesis and induced a relatively high frequency of chromosome aberrations in cultured mammalian cells. Both latter effects were reduced by the addition of catalase to solutions of the reducing agents plus Cu2+. To avoid 'False Negatives' in mutagenicity screening the use of several test subjects including mammalian cells seems to be required.
在无毒浓度下，还原剂与半胱氨酸、半胱胺、谷胱甘肽、抗坏血酸以及添加或未添加Cu2+的H2O2，均未显著增加沙门氏菌试验中的突变频率，但会引发显著的DNA修复合成，并在培养的哺乳动物细胞中诱导相对较高频率的染色体畸变。在还原剂加Cu2+的溶液中添加过氧化氢酶后，后两种效应均减弱。为避免在致突变性筛选中出现“假阴性”，似乎需要使用包括哺乳动物细胞在内的多种试验对象。
