School of Biomedical Engineering, Centre for Blood Research, University of British Columbia, Vancouver, British Columbia, Canada; email:
Integrative Oncology Department, BC Cancer Research Institute, Vancouver, British Columbia, Canada.
Annu Rev Anal Chem (Palo Alto Calif). 2022 Jun 13;15(1):221-246. doi: 10.1146/annurev-anchem-061020-111458.
Oxygen plays a fundamental role in respiration and metabolism, and quantifying oxygen levels is essential in many environmental, industrial, and research settings. Microdevices facilitate the study of dynamic, oxygen-dependent effects in real time. This review is organized around the key needs for oxygen measurement in microdevices, including integrability into microfabricated systems; sensor dynamic range and sensitivity; spatially resolved measurements to map oxygen over two- or three-dimensional regions of interest; and compatibility with multimodal and multianalyte measurements. After a brief overview of biological readouts of oxygen, followed by oxygen sensor types that have been implemented in microscale devices and sensing mechanisms, this review presents select recent applications in organs-on-chip in vitro models and new sensor capabilities enabling oxygen microscopy, bioprocess manufacturing, and pharmaceutical industries. With the advancement of multiplexed, interconnected sensors and instruments and integration with industry workflows, intelligent microdevice-sensor systems including oxygen sensors will have further impact in environmental science, manufacturing, and medicine.
氧气在呼吸和新陈代谢中起着基本作用，因此在许多环境、工业和研究环境中，定量测量氧气水平是必不可少的。微器件可促进实时研究氧气依赖性的动态效应。本综述围绕微器件中氧气测量的关键需求进行组织，包括与微加工系统的集成性；传感器的动态范围和灵敏度；在两个或三个感兴趣的区域上进行空间分辨测量以绘制氧气图；以及与多模态和多分析物测量的兼容性。在简要概述了氧气的生物读出之后，接着介绍了已经在微尺度设备中实现的氧气传感器类型和传感机制，本综述介绍了在器官芯片体外模型中的一些最新应用以及新的传感器功能，这些功能可以实现氧气显微镜、生物工艺制造和制药行业的应用。随着多路复用、互联传感器和仪器的进步以及与工业工作流程的集成，包括氧气传感器在内的智能微器件-传感器系统将在环境科学、制造和医学领域产生更大的影响。
