Department of Electrical Engineering, Centre for Biosystems, Neuroscience, and Nanotechnology, City University of Hong Kong, Hong Kong, China.
Biosensors (Basel). 2024 Aug 12;14(8):389. doi: 10.3390/bios14080389.
This study investigates the oxygen (O) consumption of single cells during changes in their migration direction. This is the first integration of nanotopographies with an O biosensor in a platform, allowing the real-time monitoring of O consumption in cells and the ability to distinguish cells migrating in the same direction from those migrating in the opposite direction. Advanced nanofabrication technologies were used to pattern nanoholes or nanopillars on grating ridges, and their effects were evaluated using fluorescence microscopy, cell migration assays, and O consumption analysis. The results revealed that cells on the nanopillars over grating ridges exhibited an enhanced migration motility and more frequent directional changes. Additionally, these cells showed an increased number of protrusions and filopodia with denser F-actin areas and an increased number of dotted F-actin structures around the nanopillars. Dynamic metabolic responses were also evident, as indicated by the fluorescence intensity peaks of platinum octaethylporphyrin ketone dye, reflecting an increased O consumption and higher mitochondria activities, due to the higher energy required in response to directional changes. The study emphasizes the complex interplay between O consumption and cell migration directional changes, providing insights into biomaterial science and regenerative medicine. It suggests innovative designs for biomaterials that guide cell migration and metabolism, advocating nanoengineered platforms to harness the intricate relationships between cells and their microenvironments for therapeutic applications.
本研究旨在探讨细胞在改变迁移方向时的耗氧量。这是首次将纳米形貌与氧生物传感器集成到一个平台中，实现了对细胞耗氧量的实时监测，并能够区分具有相同迁移方向和相反迁移方向的细胞。通过先进的纳米制造技术在光栅脊上图案化纳米孔或纳米柱，并通过荧光显微镜、细胞迁移实验和耗氧分析来评估它们的效果。结果表明，在纳米柱上的细胞在光栅脊上表现出增强的迁移运动能力和更频繁的方向变化。此外，这些细胞表现出更多的突起和丝状伪足，具有更密集的 F-肌动蛋白区域和纳米柱周围更多的点状 F-肌动蛋白结构。由于需要更高的能量来响应方向变化，还可以明显观察到动态代谢反应，如铂辛基卟啉酮染料的荧光强度峰值所示，反映出耗氧量增加和线粒体活性升高。本研究强调了耗氧量和细胞迁移方向变化之间的复杂相互作用，为生物材料科学和再生医学提供了新的见解。它为指导细胞迁移和代谢的生物材料设计提供了新思路，倡导利用纳米工程平台来利用细胞与其微环境之间的复杂关系，为治疗应用提供支持。
