Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium; VIB Center for Plant Systems Biology, 9052 Ghent, Belgium.
Ghent University, Department of Plant Biotechnology and Bioinformatics, 9052 Ghent, Belgium; VIB Center for Plant Systems Biology, 9052 Ghent, Belgium.
Plant Sci. 2022 Aug;321:111295. doi: 10.1016/j.plantsci.2022.111295. Epub 2022 Apr 20.
Modern agriculture is struggling to meet the increasing food, silage and raw material demands due to the rapid growth of population and climate change. In Arabidopsis, DA1 and DAR1 are proteases that negatively regulate cell proliferation and control organ size. DA1 and DAR1 are activated by ubiquitination catalyzed by the E3 ligase BIG BROTHER (BB). Here, we characterized the DA1, DAR1 and BB gene families in maize and analyzed whether perturbation of these genes regulates organ size similar to what was observed in Arabidopsis. We generated da1_dar1a_dar1b triple CRISPR maize mutants and bb1_bb2 double mutants. Detailed phenotypic analysis showed that the size of leaf, stem, cob, and seed was not consistently enlarged in these mutants. Also overexpression of a dominant-negative DA1 allele, resembling the da1-1 allele of Arabidopsis which has larger leaves and seeds, did not alter the maize phenotype. The mild negative effects on plant height of the DA1_bb1_bb2 mutant indicate that the genes in the DA1 pathway may control organ size in maize, albeit less obvious than in Arabidopsis.
现代农业正努力应对由于人口快速增长和气候变化带来的日益增长的粮食、青贮饲料和原材料需求。在拟南芥中，DA1 和 DAR1 是负调控细胞增殖和控制器官大小的蛋白酶。DA1 和 DAR1 通过 E3 连接酶 BIG BROTHER（BB）催化的泛素化而被激活。在这里，我们鉴定了玉米中的 DA1、DAR1 和 BB 基因家族，并分析了这些基因的扰动是否像在拟南芥中观察到的那样调节器官大小。我们生成了 da1_dar1a_dar1b 三重 CRISPR 玉米突变体和 bb1_bb2 双突变体。详细的表型分析表明，这些突变体中叶片、茎、玉米穗和种子的大小并没有一致增大。此外，过表达类似于拟南芥 da1-1 等位基因的显性负 DA1 等位基因，该等位基因具有较大的叶片和种子，并没有改变玉米表型。DA1_bb1_bb2 突变体对株高的轻微负面影响表明，DA1 途径中的基因可能控制玉米的器官大小，尽管不如拟南芥明显。
