甘薯及其两个二倍体近缘种中该基因家族的全基因组鉴定与表达分析

Genome-Wide Identification and Expression Analysis of the Gene Family in Sweet Potato and Its Two Diploid Relatives.

作者信息

Zhou Zelong, Huang Jianzhi, Wang Yuehui, He Shixiang, Yang Jing, Wang Ying, Li Wenxing, Liu Yi, Xu Ran, Li Yunhai, Wu Lian

机构信息

School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya 572025, China.

State Key Laboratory of Plant Cell and Chromosome Engineering, CAS Centre for Excellence in Molecular Plant Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.

出版信息

Int J Mol Sci. 2024 Mar 5;25(5):3000. doi: 10.3390/ijms25053000.

The gene family plays a crucial role in regulating seed and organ size in plants. The gene family has been identified in several species but has not yet been reported in sweet potatoes. In this study, nine, eleven, and seven s were identified in cultivated sweet potato (, 2n = 6x = 90) and its two diploid wild relatives, (2n = 2x = 30) and (2n = 2x = 30), respectively. The genes were classified into three subgroups based on their phylogenetic relationships with and (rice). Their protein physiological properties, chromosomal localization, phylogenetic relationships, gene structure, promoter cis-elements, and expression patterns were systematically analyzed. The qRT-PCR results showed that the expression levels of four genes, , , , and , were higher in the sweet potato leaves than in the roots, fiber roots, and stems. In our study, we provide a comprehensive comparison and further the knowledge of genes in sweet potatoes, and provide a theoretical basis for functional studies.

该基因家族在调控植物种子和器官大小方面起着关键作用。该基因家族已在多个物种中被鉴定出来，但尚未在甘薯中报道。在本研究中，分别在栽培甘薯（Ipomoea batatas，2n = 6x = 90）及其两个二倍体野生近缘种Ipomoea trifida（2n = 2x = 30）和Ipomoea triloba（2n = 2x = 30）中鉴定出9个、11个和7个该基因。基于它们与拟南芥（Arabidopsis thaliana）和水稻（Oryza sativa）的系统发育关系，将这些基因分为三个亚组。对它们的蛋白质生理特性、染色体定位、系统发育关系、基因结构、启动子顺式元件和表达模式进行了系统分析。qRT-PCR结果表明，四个基因Ib02g027600、Ib04g006800、Ib07g005800和Ib10g010000在甘薯叶片中的表达水平高于根、须根和茎。在我们的研究中，我们对甘薯中的该基因进行了全面比较并进一步了解了相关知识，为功能研究提供了理论依据。