Ng F M, Harcourt J A
Diabetologia. 1986 Dec;29(12):882-7. doi: 10.1007/BF00870144.
The in vivo and in vitro effects of a hypoglycaemic fragment of human growth hormone containing the sequence H2N-Ile-Pro-Leu-Ser-Arg-Leu-Phe-Asp-Asn-Ala-Met-Leu-COOH (hGH 4-15) on 2-deoxy-[1-14C]-D-glucose uptake in adipocytes were studied. The isolated cells from rats after a single intravenous injection of hGH 4-15 (1 mg/kg) significantly increased uptake of 2-deoxyglucose (p less than 0.005). Adipocytes from untreated rats pre-incubated with the hGH fragment (10 micrograms/ml) at 37 degrees C for 30 min also clearly showed an elevated uptake of 2-deoxyglucose in the absence and the presence of exogenous insulin. The effect of hGH 4-15 was concentration-dependent, steadily increasing with the maximum effect at 10 micrograms/ml. The present findings suggest that the enhancement of glucose transport in target tissues may be a major contributing factor to the hypoglycaemic action of the amino-terminal fragments of human growth hormone.
研究了含有序列H2N-Ile-Pro-Leu-Ser-Arg-Leu-Phe-Asp-Asn-Ala-Met-Leu-COOH（hGH 4-15）的人生长激素降血糖片段对脂肪细胞中2-脱氧-[1-14C]-D-葡萄糖摄取的体内和体外作用。单次静脉注射hGH 4-15（1 mg/kg）后从大鼠分离的细胞显著增加了2-脱氧葡萄糖的摄取（p小于0.005）。未处理大鼠的脂肪细胞在37℃下与hGH片段（10微克/毫升）预孵育30分钟，在不存在和存在外源性胰岛素的情况下，也明显显示出2-脱氧葡萄糖摄取增加。hGH 4-15的作用呈浓度依赖性，在10微克/毫升时达到最大效应并持续增加。目前的研究结果表明，靶组织中葡萄糖转运的增强可能是人生长激素氨基末端片段降血糖作用的主要促成因素。
