在腺苷脱氨酶存在的情况下，胰高血糖素对大鼠脂肪细胞胰岛素刺激的2-脱氧葡萄糖摄取的抑制作用。
Glucagon inhibition of insulin-stimulated 2-deoxyglucose uptake by rat adipocytes in the presence of adenosine deaminase.
作者信息
Green A
出版信息
Biochem J. 1983 Apr 15;212(1):189-95. doi: 10.1042/bj2120189.
Abstract
Effects of adenosine deaminase and glucagon on insulin-stimulated 2-deoxyglucose uptake by rat adipocytes are reported. (1) Adenosine deaminase (10 micrograms/ml) caused a rightward shift in the dose-response curve for the stimulation by insulin of 2-deoxyglucose uptake, but the enzyme did not alter either the basal or the maximally insulin-stimulated uptake rate. (2) In adipocytes obtained from 24 h-starved rats, glucagon inhibited the effect of insulin on 2-deoxyglucose uptake in the presence (but not in the absence) of adenosine deaminase. Basal uptake rates were unaffected. (3) Glucagon inhibited insulin-stimulated 2-deoxyglucose uptake to a greater extent in cells isolated from starved rats than in cells from fed rats. (4) Adipocytes isolated from fed and from starved rats did not differ in their capacity for degradation of 125I-labelled glucagon. The results suggest that adenosine and glucagon are regulators of insulin action in adipose tissue.
摘要
本文报道了腺苷脱氨酶和胰高血糖素对胰岛素刺激大鼠脂肪细胞摄取2-脱氧葡萄糖的影响。（1）腺苷脱氨酶（10微克/毫升）使胰岛素刺激2-脱氧葡萄糖摄取的剂量反应曲线向右移动，但该酶既不改变基础摄取率，也不改变最大胰岛素刺激摄取率。（2）在从饥饿24小时的大鼠获取的脂肪细胞中，胰高血糖素在有（但不是没有）腺苷脱氨酶存在的情况下抑制胰岛素对2-脱氧葡萄糖摄取的作用。基础摄取率不受影响。（3）与喂食大鼠的细胞相比，胰高血糖素对饥饿大鼠分离的细胞中胰岛素刺激的2-脱氧葡萄糖摄取的抑制作用更大。（4）从喂食和饥饿大鼠分离的脂肪细胞在降解125I标记的胰高血糖素的能力上没有差异。结果表明，腺苷和胰高血糖素是脂肪组织中胰岛素作用的调节因子。
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