Section for Crop Sciences, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Højbakkegård Allé 13, 2630 Tåstrup, Denmark.
Section for Crop Sciences, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Højbakkegård Allé 13, 2630 Tåstrup, Denmark.
Plant Sci. 2022 Aug;321:111323. doi: 10.1016/j.plantsci.2022.111323. Epub 2022 May 17.
Plant transformation with root oncogenic loci (rol) genes and open reading frames (ORFs) from Rhizobium rhizogenes have not yet targeted the underground root phenotype of these transformants. Hence, there is a need to develop plants with more efficient root system architecture (RSA). Here, RSA was assessed in naturally transformed (NT) and single rol/ORF Kalanchoë blossfeldiana 'Molly' lines in an aeroponic growth system combined with gene expression analysis. Three NT lines; 306, 324 and 331; exhibited better-developed RSA with longer roots and increased root biomass. In line 306, longest root was 6.3 ± 0.3 cm while WT had 4.8 ± 0.1 cm. However, root length of all overexpressing lines was ca. 30% shorter than WT. Root fresh weight of NT lines was 4.5-fold higher than WT. The expression of rolB, ∆ORF13a and ORF14 in the leaves of overexpressing lines was many folds higher than in NT lines. Increased expression of ∆ORF13a and ORF14 in leaves and roots may contribute more to a stronger compact phenotype than previously assumed. The moderate compact phenotype of NT lines combined with improved RSA compared to the overexpressing lines and WT strongly indicate that the use of R. rhizogenes has great potential to produce Kalanchoë phenotypes with enhanced RSA.
利用发根农杆菌根癌基因（rol）和开放阅读框（ORF）转化植物尚未针对这些转化体的地下根表型。因此，需要开发具有更高效根系结构（RSA）的植物。在这里，我们在气培生长系统中评估了自然转化（NT）和单个 rol/ORF 长寿花 'Molly' 系的 RSA，同时结合基因表达分析。三个 NT 系 306、324 和 331 表现出更好的 RSA，根更长，根生物量增加。在系 306 中，最长根长 6.3±0.3cm，而 WT 为 4.8±0.1cm。然而，所有过表达系的根长比 WT 短约 30%。NT 系的根鲜重比 WT 高 4.5 倍。过表达系叶片中 rolB、∆ORF13a 和 ORF14 的表达比 NT 系高几个数量级。叶片和根系中 ∆ORF13a 和 ORF14 的表达增加可能比以前假设的对更紧凑的表型贡献更大。与过表达系和 WT 相比，NT 系适度紧凑的表型与改良的 RSA 相结合，强烈表明利用发根农杆菌具有产生具有增强 RSA 的长寿花表型的巨大潜力。
