Proteomic Analysis of Proteins Related to Defense Responses in Plants Transformed with the Oncogene.

During -plant interaction, the gene is transferred into the plant genome and is stably inherited in the plant's offspring. Among the numerous effects of on plant metabolism, including the activation of secondary metabolism, its effect on plant defense systems has not been sufficiently studied. In this work, we performed a proteomic analysis of -expressing plants with particular focus on defense proteins. We found a total of 77 overexpressed proteins and 64 underexpressed proteins in transformed plants using two-dimensional gel electrophoresis and MALDI mass spectrometry. In the -transformed plants, we found a reduced amount of scaffold proteins RACK1A, RACK1B, and RACK1C, which are known as receptors for activated C-kinase 1. The proteomic analysis showed that could suppress the plant immune system by suppressing the RNA-binding proteins GRP7, CP29B, and CP31B, which action are similar to the action of type-III bacterial effectors. At the same time, plants induce the massive biosynthesis of protective proteins VSP1 and VSP2, as well as pathogenesis-related protein PR-4, which are markers of the activated jasmonate pathway. The increased contents of glutathione-S-transferases F6, F2, F10, U19, and DHAR1 and the osmotin-like defense protein OSM34 were found. The defense-associated protein PCaP1, which is required for oligogalacturonide-induced priming and immunity, was upregulated. Moreover, -transformed plants showed the activation of all components of the PYK10 defense complex that is involved in the metabolism of glucosinolates. We hypothesized that various defense systems activated by protect the host plant from competing phytopathogens and created an effective ecological niche for . A RolB → RACK1A signaling module was proposed that might exert most of the -mediated effects on plant physiology. Our proteomics data are available via ProteomeXchange with identifier PXD037959.