Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far East Branch of Russian Academy of Sciences, 159 Stoletija Str., 690022 Vladivostok, Russia.
Int J Mol Sci. 2023 Jan 18;24(3):1880. doi: 10.3390/ijms24031880.
During -plant interaction, the gene is transferred into the plant genome and is stably inherited in the plant's offspring. Among the numerous effects of on plant metabolism, including the activation of secondary metabolism, its effect on plant defense systems has not been sufficiently studied. In this work, we performed a proteomic analysis of -expressing plants with particular focus on defense proteins. We found a total of 77 overexpressed proteins and 64 underexpressed proteins in transformed plants using two-dimensional gel electrophoresis and MALDI mass spectrometry. In the -transformed plants, we found a reduced amount of scaffold proteins RACK1A, RACK1B, and RACK1C, which are known as receptors for activated C-kinase 1. The proteomic analysis showed that could suppress the plant immune system by suppressing the RNA-binding proteins GRP7, CP29B, and CP31B, which action are similar to the action of type-III bacterial effectors. At the same time, plants induce the massive biosynthesis of protective proteins VSP1 and VSP2, as well as pathogenesis-related protein PR-4, which are markers of the activated jasmonate pathway. The increased contents of glutathione-S-transferases F6, F2, F10, U19, and DHAR1 and the osmotin-like defense protein OSM34 were found. The defense-associated protein PCaP1, which is required for oligogalacturonide-induced priming and immunity, was upregulated. Moreover, -transformed plants showed the activation of all components of the PYK10 defense complex that is involved in the metabolism of glucosinolates. We hypothesized that various defense systems activated by protect the host plant from competing phytopathogens and created an effective ecological niche for . A RolB → RACK1A signaling module was proposed that might exert most of the -mediated effects on plant physiology. Our proteomics data are available via ProteomeXchange with identifier PXD037959.
在植物-病原菌互作过程中，该基因被转移到植物基因组中，并在植物后代中稳定遗传。在对植物代谢的众多影响中，包括激活次生代谢，其对植物防御系统的影响尚未得到充分研究。在这项工作中，我们对表达的拟南芥进行了蛋白质组学分析，特别关注防御蛋白。我们使用二维凝胶电泳和 MALDI 质谱法在转化植物中总共发现了 77 种过表达蛋白和 64 种低表达蛋白。在转化植物中，我们发现了已知作为激活 C 激酶 1 受体的支架蛋白 RACK1A、RACK1B 和 RACK1C 的含量减少。蛋白质组学分析表明，通过抑制 RNA 结合蛋白 GRP7、CP29B 和 CP31B，可以抑制植物免疫系统，其作用类似于 III 型细菌效应子的作用。同时，诱导大量保护性蛋白 VSP1 和 VSP2 以及与茉莉酸途径激活相关的蛋白 PR-4 的生物合成，这些都是 jasmonate 途径激活的标志物。发现谷胱甘肽-S-转移酶 F6、F2、F10、U19 和 DHAR1 以及 osmotin 样防御蛋白 OSM34 的含量增加。需要寡聚半乳糖醛酸诱导的启动和免疫的防御相关蛋白 PCaP1 上调。此外，转化植物表现出参与硫代葡萄糖苷代谢的 PYK10 防御复合物的所有成分的激活。我们假设，由 激活的各种防御系统可以保护宿主植物免受竞争的植物病原体的侵害，并为 创造了一个有效的生态位。提出了一个 RolB→RACK1A 信号模块，该模块可能发挥 介导的对植物生理学的大多数影响。我们的蛋白质组学数据可通过 ProteomeXchange 以标识符 PXD037959 获得。
