Hsa_circ_0008726 通过调节 miR-1290-LHX6 信号通路调节子痫前期滋养细胞的增殖、迁移和侵袭。
Hsa_circ_0008726 regulates the proliferation, migration, and invasion of trophoblast cells in preeclampsia through modulating the miR-1290-LHX6 signaling pathway.
机构信息
Department of Obstetrics and Gynecology, The Affiliated Hospital of Medical School of Ningbo University, Ningbo, China.
Department of Thoracic Surgery, The Affiliated Hospital of Medical School of Ningbo University, Ningbo, China.
出版信息
J Clin Lab Anal. 2022 Jul;36(7):e24540. doi: 10.1002/jcla.24540. Epub 2022 Jun 13.
BACKGROUND
Preeclampsia (PE) is a serious complication of pregnancy, with a global incidence of about 2%-8%. It is one of the important causes of morbidity and mortality among the pregnant women and perinatal infants. Circular RNA (circRNA) has been confirmed to play an important regulatory role in PE. This study aimed to evaluate the role of hsa_circ_0008726 in the occurrence and development of PE.
METHODS
The expression of hsa_circ_0008726 in PE placental tissue and blood was detected by qRT-PCR. CCK-8, wound closure, and Transwell assay were used to measure cell proliferation, migration, and invasion. Bioinformatics prediction, Western blotting, and dual-luciferase reporter gene detection were used to explore the mechanism of hsa_circ_0008726 in HTR8/SVneo cells.
RESULTS
The expression level of circ_0008726 in the placental tissue and blood samples of PE patients was significantly higher than that of normal controls. The overexpression of circ_0008726 can significantly inhibit the proliferation, migration, and invasion ability of HTR-8/SVneo cells. While the silence of circ_0008726 showed an opposite effect. Furthermore, hsa_circ_0008726 can modulate the expression of LHX6 by adsorbing miR-1290.
CONCLUSION
Hsa_circ_000872 can regulate LHX6 by adsorbing miR-1290 to inhibit PE progression, thus establishing hsa_circ_000872 as a potential target for predicting and treating PE.
背景
子痫前期（PE）是一种严重的妊娠并发症，全球发病率约为 2%-8%。它是孕产妇和围生儿发病率和死亡率的重要原因之一。环状 RNA（circRNA）已被证实在 PE 中发挥重要的调节作用。本研究旨在评估 hsa_circ_0008726 在 PE 发生和发展中的作用。
方法
采用 qRT-PCR 检测 PE 胎盘组织和血液中 hsa_circ_0008726 的表达。CCK-8、划痕愈合、Transwell 检测用于测量细胞增殖、迁移和侵袭。生物信息学预测、Western blot 和双荧光素酶报告基因检测用于探索 hsa_circ_0008726 在 HTR8/SVneo 细胞中的作用机制。
结果
PE 患者胎盘组织和血液样本中 circ_0008726 的表达水平明显高于正常对照组。circ_0008726 的过表达可显著抑制 HTR-8/SVneo 细胞的增殖、迁移和侵袭能力。而 circ_0008726 的沉默则表现出相反的效果。此外，hsa_circ_0008726 可以通过吸附 miR-1290 调节 LHX6 的表达。
结论
hsa_circ_000872 通过吸附 miR-1290 调节 LHX6 来抑制 PE 的进展，从而将 hsa_circ_000872 确立为预测和治疗 PE 的潜在靶点。
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