Laboratory of Food Chemistry, Wageningen University & Research, 6708WG Wageningen, the Netherlands.
Huvepharma NV, 2600 Berchem, Belgium.
Carbohydr Polym. 2022 Sep 1;291:119527. doi: 10.1016/j.carbpol.2022.119527. Epub 2022 Apr 26.
Previously, arabinoxylan (AX) depolymerization by dietary endo-xylanase was observed in the broiler ileum, but released arabinoxylo-oligosaccharides (AXOS) were not characterized in detail. This study aimed at extracting and identifying AXOS released in vivo in broilers, in order to delineate the influence of endo-xylanase on AX utilization. Hereto, digesta from the gizzard, ileum, ceca and excreta of broilers fed a wheat-soybean diet without (Con) or with endo-xylanase supplementation (Enz) were assessed. Soluble AX content in the ileum was higher for Enz diet (26.9%) than for Con diet (18.8%), indicating a different type and amount of AX entering the ceca. Removal of maltodextrins and fructans enabled monitoring of AX depolymerization to AXOS (Enz diet) using HPSEC-RI and HPAEC-PAD. A recently developed HILIC-MS methodology allowed AXOS (DP 4-10) identification in ileal digesta and excreta. Xylanase-induced AXOS formation coincided with decreased total tract AX recovery, which indicated improved AX hindgut utilization.
先前，在肉鸡回肠中观察到膳食纤维内切木聚糖酶对阿拉伯木聚糖（AX）的解聚作用，但未详细表征释放的阿拉伯木二糖寡糖（AXOS）。本研究旨在提取和鉴定肉鸡体内原位释放的 AXOS，以阐明内切木聚糖酶对 AX 利用的影响。为此，评估了饲喂无（Con）或添加内切木聚糖酶（Enz）的小麦-大豆日粮的肉鸡胃内容物、回肠、盲肠和粪便。Enz 日粮（26.9%）中回肠中可溶性 AX 的含量高于 Con 日粮（18.8%），这表明进入盲肠的 AX 的类型和数量不同。去除麦芽糊精和果聚糖后，使用 HPSEC-RI 和 HPAEC-PAD 可以监测到 AX 到 AXOS（Enz 日粮）的解聚。最近开发的 HILIC-MS 方法允许在回肠内容物和粪便中鉴定 AXOS（DP 4-10）。木聚糖酶诱导的 AXOS 形成与总肠道 AX 回收率降低同时发生，这表明 AX 后肠利用率得到改善。
