Department of Cuisine and Nutrition, Hubei University of Economics, Wuhan 430205, China; College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Carbohydr Polym. 2022 Sep 1;291:119569. doi: 10.1016/j.carbpol.2022.119569. Epub 2022 May 5.
The immunomodulatory effects and signalling pathways of five water-soluble yeast β-glucan fractions (WYG1-5) with different molecular weights (M) and chain conformations were investigated in RAW264.7 macrophages. All five WYG fractions were shown to increase nitric oxide (NO) production, phagocytosis activity and cytokine secretion compared with the normal group. The incubation of cells with WYG for 2 h and then with lipopolysaccharide (LPS) for 24 h showed an inhibition of NO production, phagocytosis activity, cytokine secretion, and the expression of inducible nitric oxide synthase (iNOS) and cytokine mRNA compared with the LPS group. The results showed a two-way immunomodulatory effect of WYG on inflammatory factors, with the best effect for WYG-2 having a M of 2830 × 10 g/mol and spherical conformation. Furthermore, both mitogen-activated protein kinases (MAPKs) and nuclear factor-kappa B (NF-κB) signalling pathways were triggered in the two-way immunomodulation. This study reveals the structure-activity relationship and provides a pharmacological basis for controlling inflammatory disorders with WYG.
五种不同分子量（M）和链构象的水溶性酵母β-葡聚糖（WYG1-5）的免疫调节作用及其信号通路在 RAW264.7 巨噬细胞中进行了研究。与正常组相比，所有五种 WYG 分数均显示出增加一氧化氮（NO）产生、吞噬活性和细胞因子分泌的作用。与 LPS 组相比，用 WYG 孵育细胞 2 小时，然后用脂多糖（LPS）孵育 24 小时，NO 产生、吞噬活性、细胞因子分泌以及诱导型一氧化氮合酶（iNOS）和细胞因子 mRNA 的表达均受到抑制。结果表明，WYG 对炎症因子具有双向免疫调节作用，其中 M 为 2830×10g/mol 且呈球形构象的 WYG-2 效果最佳。此外，两种丝裂原活化蛋白激酶（MAPKs）和核因子-κB（NF-κB）信号通路均在双向免疫调节中被触发。本研究揭示了结构-活性关系，并为使用 WYG 控制炎症性疾病提供了药理学基础。
