Faculty of Pharmacy and Medical Sciences, University of Petra, Amman 11196, Jordan.
Faculty of Pharmacy, Al-Zaytoonah University of Jordan, Amman 11733, Jordan.
Carbohydr Polym. 2022 Sep 1;291:119579. doi: 10.1016/j.carbpol.2022.119579. Epub 2022 May 11.
The current study aims to design a nanoparticulate system that could encapsulate insulin and improve its stability. Nanoparticles were formulated by ionic cross-linking of chitosan (CS) with carbonate divalent anions. The interaction between the two moieties was evidenced by AFM, FTIR and surface tension measurements. CS carbonate nanoparticles were prepared with different mole fractions. The mole fraction of carbonate that produced the smallest size nanoparticles and highest zeta potential (40 nm and +39 mV, respectively) was determined. Circular dichroism (CD) studies revealed that insulin conformation was not affected by CS at 20 °C. However, the studies at elevated temperatures demonstrated that CS had a role in insulin stabilization. Fluorescence spectroscopy indicated the interaction between insulin and CS carbonate. The findings from this investigation showed the potential use of CS carbonate as an insulin stabilizer and at the same time as an insulin nanocarrier system.
本研究旨在设计一种纳米颗粒系统，以包裹胰岛素并提高其稳定性。通过壳聚糖（CS）与碳酸二价阴离子的离子交联来制备纳米颗粒。通过原子力显微镜（AFM）、傅里叶变换红外光谱（FTIR）和表面张力测量来证明这两种部分之间的相互作用。用不同的摩尔分数制备了 CS 碳酸盐纳米颗粒。确定了产生最小粒径和最高 Zeta 电位（分别为 40nm 和 +39mV）的碳酸摩尔分数。圆二色性（CD）研究表明，在 20°C 时，CS 对胰岛素构象没有影响。然而，在高温下的研究表明，CS 在胰岛素稳定化方面发挥了作用。荧光光谱表明胰岛素与 CS 碳酸盐之间存在相互作用。这项研究的结果表明，CS 碳酸盐有可能被用作胰岛素稳定剂，同时也被用作胰岛素纳米载体系统。
