Mahmoud Nouf N, Hammad Ayat S, Al Kaabi Alaya S, Alawi Hend H, Khatoon Summaiya, Al-Asmakh Maha
Faculty of Pharmacy, Al-Zaytoonah University of Jordan, Amman 11733, Jordan.
Department of Biomedical Sciences, College of Health Sciences, QU Health, Qatar University, Doha 2713, Qatar.
J Funct Biomater. 2024 Sep 26;15(10):284. doi: 10.3390/jfb15100284.
Albumin-coated gold nanoparticles display potential biomedical applications, including cancer research, infection treatment, and wound healing; however, elucidating their interaction with normal cells remains an area with limited exploration. In this study, gold nanorods (GNR) were prepared and coated with bovine serum albumin (BSA) to produce GNR-BSA. The functionalized nanoparticles were characterized based on their optical absorption spectra, morphology, surface charge, and quantity of attached protein. The interaction between GNR-BSA and BSA with normal cells was investigated using human dermal fibroblasts. The cytotoxicity test indicated cell viability between ~63-95% for GNR-BSA over concentrations from 30.0 to 0.47 μg/mL and ~85-98% for BSA over concentrations from 4.0 to 0.0625 mg/mL. The impact of the GNR-BSA and BSA on cell migration potential and wound healing was assessed using scratch assay, and the modulation of cytokine release was explored by quantifying a panel of cytokines using Multiplex technology. The results indicated that GNR-BSA, at 10 μg/mL, delayed the cell migration and wound healing 24 h post-treatment compared to the BSA or the control group with an average wound closure percentage of 6% and 16% at 6 and 24 h post-treatment, respectively. Multiplex analysis revealed that while GNR-BSA reduced the release of the pro-inflammatory marker IL-12 from the activated fibroblasts 24 h post-treatment, they significantly reduced the release of IL-8 ( < 0.001), and CCL2 ( < 0.01), which are crucial for the inflammation response, cell adhesion, proliferation, migration, and angiogenesis. Although GNR-BSA exhibited relatively high cell viability towards human dermal fibroblasts and promising therapeutic applications, toxicity aspects related to cell motility and migration must be considered.
白蛋白包被的金纳米颗粒显示出潜在的生物医学应用，包括癌症研究、感染治疗和伤口愈合；然而，阐明它们与正常细胞的相互作用仍是一个探索有限的领域。在本研究中，制备了金纳米棒（GNR）并用牛血清白蛋白（BSA）进行包被以制备GNR-BSA。基于其光吸收光谱、形态、表面电荷和附着蛋白的数量对功能化的纳米颗粒进行了表征。使用人皮肤成纤维细胞研究了GNR-BSA和BSA与正常细胞之间的相互作用。细胞毒性测试表明，在30.0至0.47μg/mL的浓度范围内，GNR-BSA的细胞活力约为63-95%，在4.0至0.0625mg/mL的浓度范围内，BSA的细胞活力约为85-98%。使用划痕试验评估了GNR-BSA和BSA对细胞迁移潜力和伤口愈合的影响，并通过使用多重技术定量一组细胞因子来探索细胞因子释放的调节。结果表明，与BSA或对照组相比，在治疗后24小时，10μg/mL的GNR-BSA延迟了细胞迁移和伤口愈合，在治疗后6小时和24小时的平均伤口闭合百分比分别为6%和16%。多重分析显示，虽然在治疗后24小时GNR-BSA降低了活化成纤维细胞中促炎标志物IL-12的释放，但它们显著降低了IL-8（<0.001）和CCL2（<0.01）的释放，这两种细胞因子对于炎症反应、细胞粘附、增殖、迁移和血管生成至关重要。尽管GNR-BSA对人皮肤成纤维细胞表现出相对较高的细胞活力和有前景的治疗应用，但必须考虑与细胞运动性和迁移相关的毒性方面。
