Department of Materials Engineering, Indian Institute of Science, C.V. Raman Avenue, Bangalore 560012, India.
Department of Materials Engineering, Indian Institute of Science, C.V. Raman Avenue, Bangalore 560012, India.
Carbohydr Polym. 2022 Sep 1;291:119585. doi: 10.1016/j.carbpol.2022.119585. Epub 2022 May 7.
Injectable hydrogels exhibiting self-healing ability are promising carriers for controlled and sustained delivery in a minimally-invasive format for biomedical applications. We designed a polysaccharide-based double network hydrogel by mixing solutions of aldehyde-alginate (aAlg) and acrylic acid-chitosan (aCS) in the presence of adipic acid dihydrazide and FeCl that resulted in dual crosslinking mediated by Schiff base and ionic interactions. The hydrogel exhibited excellent thixotropic and self-healing properties with a high compressive fracture strength of ≈ 48 kPa. Encapsulated cells were viable within the hydrogel, and after their release from the degraded gel. The controlled release of Doxorubicin and Ciprofloxacin from the hydrogels established the gel as a delivery platform. The released drugs were effective in killing cancer cells or arresting the growth of both bacteria. This work presents a self-healing and injectable degradable hydrogel that may be used as a minimally-invasive platform for the delivery of drugs and cells.
具有自修复能力的可注射水凝胶有望成为一种载体，以微创的形式用于生物医学应用中的药物控制和持续释放。我们通过混合含有戊二醛的海藻酸钠（aAlg）和含有丙烯酰基的壳聚糖（aCS）溶液，在存在己二酸二酰肼和 FeCl 的条件下设计了一种基于多糖的双网络水凝胶，其通过席夫碱和离子相互作用介导双重交联。该水凝胶具有出色的触变和自修复性能，压缩断裂强度约为 48kPa。包封的细胞在水凝胶内是存活的，并且在凝胶降解后被释放出来。阿霉素和环丙沙星从水凝胶中的控制释放证实了该水凝胶是一种有效的药物输送平台。释放的药物对杀死癌细胞或抑制细菌的生长均有效。这项工作提出了一种可自修复和可注射的可降解水凝胶，可作为用于药物和细胞输送的微创平台。
