Department of Clinical Laboratory, Kameda Medical Center, Higashi-cho 929, Kamogawa-shi, Chiba, 296-8602, Japan.
Hirosaki University, Graduate School of Health Sciences, Hon-cho 66-1, Hirosaki-shi, Aomori, 036-8564, Japan.
J Med Microbiol. 2022 Jun;71(6). doi: 10.1099/jmm.0.001546.
The three species , and are difficult to distinguish, owing to their similar biochemical properties, and are often confused in medical practice. There is a scarcity of data comparing the clinical characteristics and antimicrobial susceptibility of , and . We believe that knowledge of the characteristics of each species will help in their better identification. Further, knowing the antimicrobial susceptibility of the species will help physicians in prescribing an effective treatment course for infections. This study aimed to determine the clinical characteristics and antimicrobial resistance of , and isolated from human urine samples. This study included 125 . strains isolated from human urine samples. Multiplex polymerase chain reaction was performed to identify , and . We retrospectively investigated the patient background, complications of bacteraemia, antimicrobial susceptibility and extended-spectrum β-lactamase (ESBL). We identified 84 (67.2 %), 31 (24.8 %) and 10 strains (8 .0%) of , and , respectively. There was no difference in patient background and frequency of bacteraemia complications among these species. was significantly less susceptible than to ampicillin/sulbactam (=0.03) and piperacillin (<0.01). Furthermore, (79.8 %) was less susceptible to trimethoprim/sulfamethoxazole than (96.8 %) and (100 %). There were nine ESBL-producing strains (7.2 %), all of which were . There was no difference in patient background and frequency of bacteraemia complications between , and isolated from urine. The three species showed a varying extent of antimicrobial susceptibility and ESBL production, and accurate identification is needed to understand the epidemiology of these species.
这三种物种， 和 ，由于其相似的生化特性，难以区分，在医学实践中经常混淆。比较 和 以及 的临床特征和抗菌药物敏感性的数据很少。我们相信，了解每种物种的特征将有助于更好地识别它们。此外，了解物种的抗菌药物敏感性将有助于医生为 感染制定有效的治疗方案。本研究旨在确定从人尿样中分离出的 、 和 的临床特征和抗菌药物耐药性。本研究共纳入 125 株从人尿样中分离出的 株。采用多重聚合酶链反应鉴定 、 和 。我们回顾性地调查了患者背景、菌血症并发症、抗菌药物敏感性和超广谱 β-内酰胺酶（ESBL）。我们鉴定出 84 株（67.2%）、31 株（24.8%）和 10 株（8.0%）的 、 和 。这些物种的患者背景和菌血症并发症的频率没有差异。 对氨苄西林/舒巴坦的敏感性明显低于 （=0.03）和哌拉西林（<0.01）。此外， （79.8%）对甲氧苄啶/磺胺甲恶唑的敏感性低于 （96.8%）和 （100%）。有 9 株产 ESBL 菌株（7.2%），均为 。从尿样中分离出的 、 和 ，其患者背景和菌血症并发症的频率没有差异。这三种 物种表现出不同程度的抗菌药物敏感性和 ESBL 产生，需要准确鉴定以了解这些物种的流行病学。
