Sękowska Alicja, Carrazco-Montalvo Andrés, Konechnyi Yulian
Department of Microbiology, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, 85-094 Bydgoszcz, Poland.
Department of Clinical Microbiology, Dr. A. Jurasz University Hospital No. 1 in Bydgoszcz, 85-094 Bydgoszcz, Poland.
Antibiotics (Basel). 2025 Jul 22;14(8):735. doi: 10.3390/antibiotics14080735.
is a Gram-negative, capsulated, nonmotile, facultative anaerobic rod. It is one of the species belonging to the . The objective of this study was to gain insights into the antimicrobial resistance and virulence of strains isolated from clinical samples from oncologic patients. Strain identification was performed using a mass spectrometry method. Whole genome sequencing was conducted for all analyzed strains. Antimicrobial susceptibility was determined using an automated method. The presence of antimicrobial resistance mechanisms and genes encoding extended-spectrum beta-lactamases (ESBL) was assessed using the double-disc synergy test and genotypic methods. All isolates were identified as using mass spectrometry and whole genome sequencing (WGS). All isolates were ESBL-positive, and two of them harbored the gene. In our study, the gene was detected in all strains. Genome sequence analysis of the isolates revealed the presence of virulence factor genes, including , , , , and . Two different plasmids, IncFIB(K) and IncFII, were identified in all of the analyzed strains. The detected virulence factors suggest the ability of the bacteria to survive in the environment and infect host cells. All isolates demonstrated in vitro susceptibility to carbapenems. Further studies are needed to confirm whether multidrug-resistant strains represent an important pathogen in infections among oncologic patients.
是一种革兰氏阴性、有荚膜、无动力、兼性厌氧的杆菌。它是属于……的物种之一。本研究的目的是深入了解从肿瘤患者临床样本中分离出的……菌株的抗菌耐药性和毒力。使用质谱法进行菌株鉴定。对所有分析菌株进行全基因组测序。使用自动化方法测定抗菌药物敏感性。使用双碟协同试验和基因分型方法评估抗菌耐药机制和编码超广谱β-内酰胺酶（ESBL）的基因的存在情况。所有分离株通过质谱法和全基因组测序（WGS）鉴定为……。所有分离株均为ESBL阳性，其中两株携带……基因。在我们的研究中，所有菌株均检测到……基因。对……分离株的基因组序列分析显示存在毒力因子基因，包括……、……、……、……和……。在所有分析的……菌株中鉴定出两种不同的质粒，IncFIB(K)和IncFII。检测到的毒力因子表明细菌在环境中生存和感染宿主细胞的能力。所有分离株在体外对碳青霉烯类药物敏感。需要进一步研究以确认多重耐药的……菌株是否是肿瘤患者感染中的重要病原体。
