Whole Genome Sequencing of Strains Isolated from Patients with Cancer.

is a Gram-negative, capsulated, nonmotile, facultative anaerobic rod. It is one of the species belonging to the . The objective of this study was to gain insights into the antimicrobial resistance and virulence of strains isolated from clinical samples from oncologic patients. Strain identification was performed using a mass spectrometry method. Whole genome sequencing was conducted for all analyzed strains. Antimicrobial susceptibility was determined using an automated method. The presence of antimicrobial resistance mechanisms and genes encoding extended-spectrum beta-lactamases (ESBL) was assessed using the double-disc synergy test and genotypic methods. All isolates were identified as using mass spectrometry and whole genome sequencing (WGS). All isolates were ESBL-positive, and two of them harbored the gene. In our study, the gene was detected in all strains. Genome sequence analysis of the isolates revealed the presence of virulence factor genes, including , , , , and . Two different plasmids, IncFIB(K) and IncFII, were identified in all of the analyzed strains. The detected virulence factors suggest the ability of the bacteria to survive in the environment and infect host cells. All isolates demonstrated in vitro susceptibility to carbapenems. Further studies are needed to confirm whether multidrug-resistant strains represent an important pathogen in infections among oncologic patients.