Department of Nutrition and Dietetics, Faculty of Health Sciences, Hakkari University, Hakkari, Turkey
Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Harran University, Sanliurfa, Turkey
New Microbiol. 2022 Apr;45(2):124-129.
Carbapenem-resistant Klebsiella pneumoniae is associated with high morbidity and mortality, and capsule serotypes make treatment difficult. The aim of this study is to investigate the relationship between colistin resistance and capsule types in carbapenem-resistant K. pneumoniae isolates. In 2018- 2020, we conducted our study with 115 carbapenem-resistant K. pneumoniae strains diagnosed by matrix-mediated laser desorption ionization time-of-flight mass spectrometry method (MALDI-TOF MS; Bruker Daltonics, Germany). Colistin sensitivities were determined by using DxM MicroScan WalkAway System (Beckman Coulter, ABD) automated system and were then verified by liquid microdilution (MIC). Capsule serotypes were investigated by conventional polymerase chain reaction (PCR) method. Among the carbapenem resistant K. pneumoniae isolates, 42% (48) were resistant to colistin and 58% (67) were susceptible to colistin. In the K. pneumoniae isolates with colistin resistance 33% (16) K5, 13% (6) K2, 8% (4) K20 4% (2) K1 and 2% (1) K54 and K57 capsule serotypes were found, while in the K. pneumoniae isolates with colistin susceptible 12% (8) K5, 4% (3) K2, 3% (2) K20, 1.5% (1) K1 and K54 capsule serotypes were found. Serotype K5 was very frequent in isolates collected from patients with urinary tract diseases. The resistance profile data obtained from the present study can serve as an information base to understand the infection pattern prevailing in the hospital.
耐碳青霉烯类肺炎克雷伯菌与高发病率和死亡率相关，且荚膜血清型使治疗变得困难。本研究旨在探讨耐碳青霉烯类肺炎克雷伯菌分离株中多黏菌素耐药与荚膜型之间的关系。在 2018 年至 2020 年期间，我们通过基质辅助激光解吸电离飞行时间质谱法（MALDI-TOF MS；德国布鲁克道尔顿公司）对 115 株经诊断为耐碳青霉烯类肺炎克雷伯菌的菌株进行了研究。采用 DxM MicroScan WalkAway 系统（贝克曼库尔特公司，美国）自动化系统测定多黏菌素敏感性，并用液体微量稀释法（MIC）进行验证。荚膜血清型通过常规聚合酶链反应（PCR）法进行检测。在耐碳青霉烯类肺炎克雷伯菌分离株中，42%（48 株）对多黏菌素耐药，58%（67 株）对多黏菌素敏感。在多黏菌素耐药的肺炎克雷伯菌分离株中，发现了 33%（16 株）K5、13%（6 株）K2、8%（4 株）K20、4%（2 株）K1 和 2%（1 株）K54 和 K57 荚膜血清型，而在多黏菌素敏感的肺炎克雷伯菌分离株中，发现了 12%（8 株）K5、4%（3 株）K2、3%（2 株）K20、1.5%（1 株）K1 和 K54 荚膜血清型。在从患有尿路感染疾病的患者中采集的分离株中，血清型 K5 非常常见。本研究获得的耐药谱数据可作为了解医院流行感染模式的信息基础。
