Graduate School of Environmental Studies, Tohoku University, 6-6-11 Aramaki Aoba, Aoba, Sendai, Miyagi 980-8579, Japan.
Center for Basic Education, Faculty of Engineering, Graduate Faculty of Interdisciplinary Research, University of Yamanashi, 4-3-11 Takeda, Kofu 400-8511, Japan.
Anal Chem. 2022 Jun 28;94(25):8857-8866. doi: 10.1021/acs.analchem.1c05354. Epub 2022 Jun 14.
In this study, a carbon paste filling method was proposed as a simple strategy for fabricating high-density bipolar electrode (BPE) arrays for bipolar electrochemical microscopy (BEM). High spatiotemporal resolution imaging was achieved using the fabricated BPE array. BEM, which is an emerging microscopic system in recent years, achieves label-free and high spatiotemporal resolution imaging of molecular distributions using high-density BPE arrays and electrochemiluminescence (ECL) signals. We devised a simple method to fabricate a BPE array by filling a porous plate with carbon paste and succeeded in fabricating a high-density BPE array (15 μm pitch). After a detailed observation of the surface of the BPE array using a scanning electron microscope, the basic electrochemical and ECL emission characteristics were evaluated using potassium ferricyanide solution as a sample solution. Moreover, inflow imaging of the sample molecules was conducted to evaluate the imaging ability of the prepared BPE array. In addition, Prussian Blue containing carbon ink was applied to the sample solution side of the BPE array to provide catalytic activity to hydrogen peroxide, and the quantification and inflow imaging of hydrogen peroxide by ECL signals was achieved. This simple fabrication method of the BPE array can accelerate the research and development of BEM. Furthermore, hydrogen peroxide imaging by BEM is an important milestone for achieving bioimaging with high spatiotemporal resolution such as biomolecule imaging using enzymes.
在这项研究中，提出了一种碳糊填充方法，作为一种简单的策略，用于制造高密度双极电极（BPE）阵列用于双极电化学显微镜（BEM）。使用所制造的 BPE 阵列实现了高时空分辨率成像。BEM 是近年来新兴的微观系统，使用高密度 BPE 阵列和电化学发光（ECL）信号实现分子分布的无标记和高时空分辨率成像。我们设计了一种简单的方法，通过用碳糊填充多孔板来制造 BPE 阵列，并成功制造了高密度 BPE 阵列（15 μm 间距）。使用扫描电子显微镜对 BPE 阵列的表面进行详细观察后，使用铁氰化钾溶液作为样品溶液评估了基本的电化学和 ECL 发射特性。此外，进行了样品分子的流入成像，以评估制备的 BPE 阵列的成像能力。此外，将含普鲁士蓝的碳墨水施加到 BPE 阵列的样品溶液侧，以提供对过氧化氢的催化活性，并通过 ECL 信号实现了过氧化氢的定量和流入成像。这种 BPE 阵列的简单制造方法可以加速 BEM 的研究和开发。此外，BEM 的过氧化氢成像对于实现高时空分辨率的生物成像，例如使用酶进行生物分子成像，是一个重要的里程碑。
