Initiating an anaerobic ammonium oxidation reactor by inoculation with starved anaerobic ammonium oxidation sludge and modified carriers.

Prolonged starved anammox sludge (SAS) obtained during initial rejuvenation was inoculated into a reactor together with activated sludge (AS), anaerobic granular sludge (AGS) and modified carriers consisting of honeycomb carrier with high biological interception and activated carbon carrier with high adsorption performance. SAS accounted for 5% of the inoculated sludge. The anammox process was started and operated at around 25℃. After 160 days, the nitrogen loading rate and nitrogen removal rate reached 1.12 kgN·m·d and 0.97 kgN·m·d, respectively. Obvious red anammox biofilms were observed on the modified carriers. Microbial community analysis showed that the relative abundance of anammox bacteria increased from < 0.1% to 22.96%. Candidatus Jettenia and Candidatus Brocadia were the dominating anammox species. This work demonstrates the potential to reuse SAS to improve the start-up efficiency of anammox reactors, which makes good economic sense.