Enhancement of Partial Nitrification-Anaerobic Ammonia Oxidation in SBR Reactors via Surface-Modified Polyurethane Sponge Biofilm Carrier.

The partial nitrification-anammox process offers a cost-effective, energy-efficient, and environmentally sustainable approach for nitrogen removal in wastewater treatment. However, its application under low ammonia nitrogen conditions faces operational challenges including prolonged start-up periods and excessive nitrite oxidation. This study employed a strategy combining polyurethane surface positive charge enhancement and zeolite loading to develop a carrier capable of microbial enrichment and inhibition of nitrate generation, aiming to initiate the partial nitrification-anammox process in a sequencing batch reactor. Operational results demonstrate that the modified carrier enabled the reactor to achieve a total nitrogen removal efficiency of 78%, with the effluent nitrate nitrogen reduced to 6.03 mg-N/L, successfully initiating the partial nitrification-anammox process. The modified carrier also exhibited accelerated biofilm proliferation (both suspended and attached biomass increased). Additionally, 16S rRNA revealed a higher relative abundance of typical anammox bacteria Brocadia in the biofilm of the modified carrier compared to the original carrier, alongside a decline in nitrifying genera, such as . These microbial shifts effectively suppressed excessive nitrite oxidation, limited nitrate accumulation, and sustained efficient nitrogen removal throughout the reactor's operation.