Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Belgrade, Serbia.
Faculty of Chemistry, University of Belgrade, Belgrade, Serbia.
Phytochem Anal. 2022 Aug;33(6):961-970. doi: 10.1002/pca.3151. Epub 2022 Jun 14.
Ramonda serbica and R. nathaliae are resurrection plants that have the remarkable ability to survive the complete desiccation of their vegetative organs (i.e. leaves, stem, roots) during periods of drought and rapidly revive when rewatered and rehydrated.
To investigate metabolic changes in R. serbica and R. nathaliae during their desiccation and recovery process METHODS: Proton nuclear magnetic resonance ( H-NMR) and gas chromatography-mass spectrometry (GC-MS)-based metabolomics approach coupled with multivariate data analysis was utilised to identify the metabolomes of the plants from 90 biological replicates.
Sucrose and the polyphenolic glycoside myconoside were predominant in almost equal amounts in all samples studied, regardless of their water content at sampling. During the dehydration process, a decrease in the relative content of fructose, galactose, and galactinol was observed while the contents of those metabolites were preserved in the partially rehydrated plants. Raffinose and myo-inositol were accumulated in dry samples.
Using H-NMR and GC-MS as two complementary analytical platforms provided a more complete picture of the metabolite composition for investigation of the desiccation and recovery process in resurrection plants.
塞尔维亚柳兰和纳塔莉亚柳兰是复苏植物，它们具有在干旱期间完全使营养器官（即叶子、茎、根）脱水并在重新浇水和再水合时迅速复活的非凡能力。
研究塞尔维亚柳兰和纳塔莉亚柳兰在脱水和恢复过程中的代谢变化。
利用基于质子核磁共振（ 1H-NMR）和气相色谱-质谱（GC-MS）的代谢组学方法结合多变量数据分析来鉴定来自 90 个生物学重复的植物代谢组。
在研究的所有样品中，蔗糖和多酚糖苷麦角酰苷的含量几乎相等，无论其在采样时的含水量如何。在脱水过程中，观察到果糖、半乳糖和半乳糖醇的相对含量下降，而部分再水合植物中这些代谢物的含量得以保留。在干燥样品中积累了棉子糖和肌醇。
使用 1H-NMR 和 GC-MS 作为两种互补的分析平台，为研究复苏植物的脱水和恢复过程中代谢物组成提供了更全面的图景。
