牛血清免疫球蛋白富集组分中针对SARS-CoV-2变异株刺突蛋白的抗体。
Antibodies against spike protein of SARS-CoV-2 variants in bovine whey IgG enriched fraction.
作者信息
Oshiro Satoshi, Mizutani Naeko, Tada Tatsuya, Sekiguchi Jun-Ichiro, Takahashi Masao, Kirikae Teruo
机构信息
Department of Microbiology, Juntendo University School of Medicine, Tokyo, Japan.
Microbiology Research Division, Kohjin Bio Co., Ltd., Saitama, Japan.
出版信息
Int Dairy J. 2022 Oct;133:105436. doi: 10.1016/j.idairyj.2022.105436. Epub 2022 Jun 10.
Bovine whey IgG enriched fraction contains IgG antibodies against bacterial and viral pathogens, including antibodies against the spike protein [amino acids (aa) 1-1274] of SARS-CoV-2 Wuhan strain (2019-nCoV WHU01). To date, 13 SARS-CoV-2 variants have been identified, including gamma, delta, kappa, and omicron, which contain 10, eight, seven, and over 30 mutations in the spike protein, respectively. We investigated whether bovine whey IgG enriched fraction contains antibodies against spike proteins of these variants, specifically recombinant partial length spike proteins (aa 177-512, aa 509-685, aa 177-324, aa 250-410 and aa 387-516) of these variants. Direct enzyme-linked immunosorbent assays revealed bovine whey IgG enriched fraction contained antibodies against all recombinant spike proteins of these variants with highest reactivity against aa 177-512 region of omicron spike protein. These results indicate bovine whey IgG enriched fraction contains antibodies against spike proteins of several SARS-CoV-2 variants, including omicron.
富含牛血清免疫球蛋白G的组分含有针对细菌和病毒病原体的免疫球蛋白G抗体，包括针对严重急性呼吸综合征冠状病毒2（SARS-CoV-2）武汉株（2019-nCoV WHU01）刺突蛋白[氨基酸（aa）1-1274]的抗体。迄今为止，已鉴定出13种SARS-CoV-2变体，包括伽马、德尔塔、卡帕和奥密克戎，它们在刺突蛋白中分别含有10个、8个、7个和30多个突变。我们研究了富含牛血清免疫球蛋白G的组分是否含有针对这些变体刺突蛋白的抗体，特别是这些变体的重组部分长度刺突蛋白（aa 177-512、aa 509-685、aa 177-324、aa 250-410和aa 387-516）。直接酶联免疫吸附测定显示，富含牛血清免疫球蛋白G的组分含有针对这些变体所有重组刺突蛋白的抗体，对奥密克戎刺突蛋白的aa 177-512区域反应性最高。这些结果表明，富含牛血清免疫球蛋白G的组分含有针对几种SARS-CoV-2变体刺突蛋白的抗体，包括奥密克戎。
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