Guangdong Academy of Agricultural Sciences, Sericultural & Agri-Food Research Institute/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou, China.
Guangxi Rongshui Furongbei Jiangyuan Agricultural Development Co., Ltd, Liuzhou, China.
J Food Biochem. 2022 Oct;46(10):e14270. doi: 10.1111/jfbc.14270. Epub 2022 Jun 15.
The effective components of mulberry leaf polyphenols (MLPs) should be absorbed and transported by the intestinal cells before regulating lipid metabolism. The Caco-2 intestinal epithelial cell and 3 T3-L1 adipocytes were coupled to screen the effective components of MLPs that are being absorbed and transported by intestinal cells. The regulation and molecular mechanism by which the effective components affect adipogenesis were analyzed in this study. Among the 12 main components identified, five main compounds were well absorbed with Papp in the order of benzoic acid > chlorogenic acid > astragaloside > hyperoside > rutin. Chlorogenic acid and benzoic acid were mainly absorbed through passive diffusion, while rutin, astragaloside, and hyperoside were mainly by active transport, of which chlorogenic and rutin absorption were mediated by the efflux protein, P-glycoprotein (P-pg). Based on the transport volume of 2 mg/ml MLPs within 2 h, 25% of the maximum transported MLPs (TMLPs) was a safe concentration for 3 T3-L1 preadipocytes. Except for astragaloside, the other four components showed a significant inhibitory effect on lipid droplets, TG and TC, and chlorogenic acid and benzoic acid had the strongest effect. Additionally, we observed a synergistic effect as TMLPs were the most effective. We hypothesized that TMLPs, chlorogenic acid and benzoic acid suppressed adipogenesis and regulated lipid metabolism by inhibiting PPAR-γ, C/EBP-α, and FAS mRNA while promoting ADIPO and Leptin mRNA expression. PRACTICAL APPLICATIONS: The absorption and adipogenesis inhibition effect of mulberry leaf phenolics were evaluated in this study. The results provided guideline for the development of functional foods in regulating lipid metabolism.
桑叶多酚（MLPs）的有效成分在调节脂代谢之前应被肠细胞吸收和转运。本研究将 Caco-2 肠上皮细胞和 3T3-L1 脂肪细胞偶联，筛选被肠细胞吸收和转运的 MLPs 的有效成分。分析了有效成分调节脂肪生成的作用及其分子机制。在所鉴定的 12 种主要成分中，有 5 种主要化合物的 Papp 良好，其顺序为苯甲酸＞绿原酸＞黄芪甲苷＞芦丁＞槲皮素。绿原酸和苯甲酸主要通过被动扩散吸收，而芦丁、黄芪甲苷和槲皮素主要通过主动转运吸收，其中绿原酸和芦丁的吸收由外排蛋白 P-糖蛋白（P-pg）介导。基于 2 h 内 2 mg/ml MLPs 的转运量，25%的最大转运 MLPs（TMLPs）是 3T3-L1 前脂肪细胞的安全浓度。除黄芪甲苷外，其余 4 种成分对脂滴、TG 和 TC 均有明显的抑制作用，其中绿原酸和苯甲酸的作用最强。此外，我们观察到 TMLPs 最为有效，存在协同作用。我们假设 TMLPs、绿原酸和苯甲酸通过抑制 PPAR-γ、C/EBP-α 和 FAS mRNA 表达，同时促进 ADIPO 和 Leptin mRNA 表达，抑制脂肪生成，调节脂代谢。
本研究评价了桑叶酚的吸收和抑制脂肪生成作用。结果为调节脂代谢的功能性食品开发提供了指导。
