Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul-UFRGS, Av. Ipiranga 2752, 90610-000 Porto Alegre, RS, Brazil.
Plant Foods Hum Nutr. 2012 Jun;67(2):156-61. doi: 10.1007/s11130-012-0289-x.
Leaves of Ilex paraguariensis are used to prepare a tea known as maté which is a common beverage in several South American countries. The ethanol extract was fractionated to identify the compounds responsible for the anti-adipogenic activity in 3T3-L1 cells. Extracts of both fresh and dried maté leaves were subjected to column chromatography using molecular permeation to obtain the saponin (20 % yields) and the polyphenol extracts (40 % yields) from the fresh and dried leaves. The phenolic content was determined using high-performance liquid chromatography analysis and the Folin-Ciocalteau method. Also, maté extracts (50 μg/ml to 1,000 μg/ml) did not display citotoxicity using MTT. The polyphenol extract from the dried leaves was the most effective (50 μg/ml) in the inhibition of triglyceride accumulation in 3T3-L1 adipocytes, and rutin (100 μg/ml) likely accounted for a large portion of this activity. Additionally, maté extracts had a modulatory effect on the expression of genes related to the adipogenesis as PPARγ2, leptin, TNF-α and C/EBPα.
巴拉圭冬青的叶子被用来制备一种茶，称为马黛茶，这是南美洲几个国家的常见饮料。将乙醇提取物进行分段，以鉴定出对 3T3-L1 细胞具有抗脂肪生成活性的化合物。使用分子渗透对新鲜和干燥的马黛茶叶子的提取物进行柱层析，从新鲜和干燥的叶子中获得皂苷（20%的产率）和多酚提取物（40%的产率）。使用高效液相色谱分析和 Folin-Ciocalteau 法测定酚类含量。此外，马黛提取物（50μg/ml 至 1000μg/ml）在使用 MTT 时没有显示细胞毒性。来自干叶的多酚提取物在抑制 3T3-L1 脂肪细胞中甘油三酯积累方面最有效（50μg/ml），而芦丁（100μg/ml）可能对此活性起很大作用。此外，马黛提取物对与脂肪生成相关的基因（PPARγ2、瘦素、TNF-α和 C/EBPα）的表达具有调节作用。
