Wahl R L, Kimmel K A, Beierwaltes W H, Carey T E
Hybridoma. 1987 Apr;6(2):111-9. doi: 10.1089/hyb.1987.6.111.
Squamous cell carcinomas (SCC) of the head and neck are common and aggressive tumors. A murine IgG 2aK monoclonal antibody (A9) reactive with most SCCs was radiolabeled with I-131 and tested for localization and imaging ability in groups of nude mice bearing SCC tumors derived from two patients with head and neck cancer (UM-SCC-2 and UM-SCC-11B). The mice also received I-125 labeled isotype-matched control antibody UPC-10 for double-label counting. Animals were imaged at multiple time points post-injection and sacrificed 7-10 days later for assessment of the tissue distribution of the radiopharmaceuticals. In general, the tumors were visualized satisfactorily on gamma camera images of both the UM-SCC-2 and UM-SCC-11B tumor-bearing animals. While localization of the A9 antibody was mainly due to antibody specificity in the UM-SCC-2 tumor-bearing animals, there was also a large component of non-specific antibody localization in the UM-SCC-11B bearing animals. This may be due to greater central fluid space and or necrosis in the UM-SCC-11B tumors. This study demonstrates the feasibility of imaging human-derived squamous cell carcinomas with radiolabeled monoclonal antibody A9 and holds forth the possibility of therapy of this radiosensitive tumor in a like manner.
头颈部鳞状细胞癌（SCC）是常见的侵袭性肿瘤。一种与大多数SCC反应的鼠源IgG 2aK单克隆抗体（A9）用I-131进行放射性标记，并在两组携带源自两名头颈癌患者（UM-SCC-2和UM-SCC-11B）的SCC肿瘤的裸鼠中测试其定位和成像能力。这些小鼠还接受了I-125标记的同型匹配对照抗体UPC-10用于双标记计数。在注射后多个时间点对动物进行成像，并在7-10天后处死以评估放射性药物的组织分布。总体而言，在携带UM-SCC-2和UM-SCC-11B肿瘤的动物的γ相机图像上，肿瘤均能得到满意的显影。在携带UM-SCC-2肿瘤的动物中，A9抗体的定位主要归因于抗体特异性，而在携带UM-SCC-11B肿瘤的动物中，也存在大量非特异性抗体定位。这可能是由于UM-SCC-11B肿瘤中更大的中央液腔和/或坏死。本研究证明了用放射性标记的单克隆抗体A9对人源鳞状细胞癌进行成像的可行性，并提出了以类似方式治疗这种放射敏感性肿瘤的可能性。
