Niedermair Tanja, Bhatt Meet, Babel Maximilian, Feustel Moritz, Mamilos Andreas, Schweikl Helmut, Ferstl Gerlinde, Hofman Paul, Brochhausen Christoph
Institute of Pathology, University Regensburg, University of Regensburg, Regensburg, Germany.
Central Biobank Regensburg, University Clinic and University of Regensburg, Regensburg, Germany.
Biopreserv Biobank. 2023 Apr;21(2):149-157. doi: 10.1089/bio.2022.0002. Epub 2022 Jun 14.
One major goal of biobanks is to provide the best possible biospecimen quality for research use. This can be achieved, notably in accredited structures, by using standardized procedures for collection, processing, and storage of biosamples and associated data. Since tissue samples of a clinical biobank are commonly collected at surgical theaters in satellite locations or hospitals in remote areas, adequate temporary storage of the biosample is mandatory to maintain optimal sample quality. In cases where immediate snap freezing of the collected material is possible, interim storage of the samples in portable dewars filled with liquid nitrogen (LN) is a widely used method. Therefore, the ideal dewar size and maximum storage time need to be considered to maintain an optimal biospecimen quality. In addition, the nature of the cryotube material is an important aspect for keeping the biosample safe while storing it in LN. The objective of this study was to test different dewar vessels with respect to LN volume and consumption and to analyze the impact of LN contact on cryotube material through scanning electron microscopy.
生物样本库的一个主要目标是为研究提供尽可能优质的生物样本。这可以通过使用生物样本及相关数据的标准化采集、处理和存储程序来实现，尤其是在经过认证的机构中。由于临床生物样本库的组织样本通常在偏远地区的卫星地点手术室或医院采集，因此必须对生物样本进行适当的临时存储，以保持最佳样本质量。在可能对采集的材料立即进行速冻的情况下，将样本临时存放在装满液氮（LN）的便携式杜瓦瓶中是一种广泛使用的方法。因此，需要考虑理想的杜瓦瓶尺寸和最长存储时间，以保持最佳生物样本质量。此外，冷冻管材料的性质是将生物样本存储在液氮中时确保其安全的一个重要方面。本研究的目的是测试不同杜瓦瓶在液氮体积和消耗方面的情况，并通过扫描电子显微镜分析液氮接触对冷冻管材料的影响。
