DNA 损伤时 R 环复合物的邻近连接检测。
Proximity Ligation Assay for Detection of R-Loop Complexes upon DNA Damage.
机构信息
Sir William Dunn School of Pathology, University of Oxford, Oxford, UK.
出版信息
Methods Mol Biol. 2022;2528:289-303. doi: 10.1007/978-1-0716-2477-7_19.
PMID:35704199
Abstract
In situ Proximity Ligation Assay (PLA ) can be used to detect the close proximity (less than ~40 nm) of two biological molecules of interest in cells. Here we report the application of this method for the specific detection of R-loop interacting proteins and RNA modifications in close proximity to R-loops in non-damage and ionizing radiation (IR) induced DNA damage conditions.
摘要
原位邻近连接分析（PLA）可用于检测细胞中两个感兴趣的生物分子的近距离（小于~40nm）。本文报告了该方法在非损伤和电离辐射（IR）诱导的 DNA 损伤条件下，特异性检测 R 环相互作用蛋白和 RNA 修饰在 R 环附近的应用。
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