A long noncoding RNA promotes parasite differentiation in African trypanosomes.

The parasite causes African sleeping sickness that is fatal to patients if untreated. Parasite differentiation from a replicative slender form into a quiescent stumpy form promotes host survival and parasite transmission. Long noncoding RNAs (lncRNAs) are known to regulate cell differentiation in other eukaryotes. To determine whether lncRNAs are also involved in parasite differentiation, we used RNA sequencing to survey the genome, identifying 1428 previously uncharacterized lncRNA genes. We find that lncRNA is a key regulator that promotes parasite differentiation into the quiescent stumpy form. This function is promoted by a small nucleolar RNA encoded within the lncRNA. binds to messenger RNAs of at least two stumpy regulatory genes, promoting their expression. overexpression reduces parasitemia in infected mice. Our analyses suggest that lncRNAs modulate parasite-host interactions and provide a mechanism by which regulates cell differentiation in trypanosomes.