Department of Life, Health and Environmental Sciences, University of L'Aquila, 67100 L'Aquila, Italy.
Department of Biology and Biotechnology "Charles Darwin", Sapienza University of Rome, 00185 Rome, Italy.
Mol Plant Microbe Interact. 2022 Oct;35(10):881-886. doi: 10.1094/MPMI-05-22-0113-TA. Epub 2022 Sep 19.
Oligogalacturonide (OG)-oxidase 1 (OGOX1) and cellodextrin (CD)-oxidase (CELLOX) are plant berberine bridge enzyme-like oligosaccharide oxidases that oxidize OGs and CDs, cell-wall fragments with the nature of damage-associated molecular patterns. The oxidation of OGs and CDs attenuates their elicitor activity and concomitantly releases HO. By using a multiple enzyme-based assay, we demonstrate that the HO generated downstream of the combined action between a fungal polygalacturonase and OGOX1 or an endoglucanase and CELLOX can be directed by plant peroxidases (PODs) either towards a reaction possibly involved in plant defense, such as the oxidation of monolignol or a reaction possibly involved in a developmental event, such as the oxidation of auxin (indole-3-acetic acid), pointing to OGOX1 and CELLOX as enzymatic transducers between microbial glycoside hydrolases and plant PODs. [Formula: see text] Copyright © 2022 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
寡聚半乳糖醛酸酶（OG）氧化酶 1（OGOX1）和纤维二糖氧化酶（CELLOX）是植物小檗碱桥酶样寡糖氧化酶，可氧化具有损伤相关分子模式性质的寡糖和纤维二糖细胞壁片段。OG 和 CDs 的氧化可降低其激发子活性，并同时释放 HO。通过使用多种酶基测定法，我们证明了真菌多聚半乳糖醛酸酶与 OGOX1 或内切葡聚糖酶与 CELLOX 联合作用下游产生的 HO 可以被植物过氧化物酶（POD）引导至可能参与植物防御的反应，如单酚的氧化，或可能参与发育事件的反应，如生长素（吲哚-3-乙酸）的氧化，这表明 OGOX1 和 CELLOX 是微生物糖苷水解酶与植物 POD 之间的酶转导物。[公式：见正文] 版权所有 © 2022 作者。这是一篇在 CC BY-NC-ND 4.0 国际许可下发布的开放获取文章。
