Bucht E, Arver S, Sjöberg H E
Int J Androl. 1986 Oct;9(5):341-7. doi: 10.1111/j.1365-2605.1986.tb00896.x.
Semen samples were obtained from 4 healthy males on two occasions. One of the samples was liquefied at room temperature for 20 min while the other was diluted immediately with buffer to suppress proteolysis. A pool of immunoextracted calcitonin from liquefied and diluted samples, respectively, was subjected to gel chromatography in a separate experiment. In lyophilized samples the majority of immunoreactive calcitonin (CT) had an approximate molecular weight (Mr) of 10 kilodaltons (Kd) and immunoreactive PDN-21 was also present in the fractions. In diluted ejaculates CT was composed of larger species as well as the 10 Kd form. PDN-21 was present in fractions containing the 10 Kd form but could not be detected in the peaks of larger molecular weight, probably because of lower sensitivity of the PDN-21 assay compared to the CT assay. Our finding of CT and PDN-21 in the same fractions after immunoextraction with CT antibodies suggests the presence of proforms of CT in human seminal fluid.
从4名健康男性身上分两次采集精液样本。其中一份样本在室温下液化20分钟，另一份则立即用缓冲液稀释以抑制蛋白水解。在另一个单独的实验中，分别对液化和稀释后的样本进行免疫提取降钙素，然后将提取后的降钙素合并进行凝胶色谱分析。在冻干样本中，大部分免疫反应性降钙素（CT）的近似分子量（Mr）为10千道尔顿（Kd），并且免疫反应性PDN-21也存在于这些组分中。在稀释后的射精样本中，CT由较大分子形式以及10 Kd形式组成。PDN-21存在于含有10 Kd形式的组分中，但在较大分子量的峰中未检测到，这可能是因为与CT检测相比，PDN-21检测的灵敏度较低。我们在用CT抗体进行免疫提取后，在相同组分中发现CT和PDN-21，这表明人精液中存在CT的前体形式。
