Laboratory of Veterinary Physiology, Faculty of Veterinary Medicine, Okayama University of Science, Ehime, Japan.
J Vet Med Sci. 2022 Aug 1;84(8):1065-1073. doi: 10.1292/jvms.22-0141. Epub 2022 Jun 15.
Pituitary gonadotropin secretion is regulated by several pituitary factors as well as GnRH and ovarian hormones. To elucidate the regulatory mechanisms of pituitary gonadotropin secretions, we observed changes in the mRNA levels of pituitary factors, namely annexin A1 (Anxa1) and Anxa5, inhibin/activin subunits, follistatin (Fst), and vitamin D receptor (Vdr), in female rat pituitary glands during the estrous cycle. Additionally, levels of LHβ subunit (Lhb), FSHβ subunit (Fshb), and GnRH receptor (Gnrh-r) mRNA were examined. During proestrus, Anxa1, Anxa5, Vdr, and inhibin α-subunit (Inha) exhibited the lowest levels, while during estrus, activin βB-subunit (Actbb), Lhb, and Gnrh-r were the lowest. Moreover, Fshb exhibited the highest value during metestrus, whereas Fst did not differ significantly. Correlation analyses revealed 16 statistically significant gene combinations. In particular, four combinations, namely Anxa5 and Inha, Anxa5 and Actbb, Inha and Vdr, and Inha and Actbb, were highly significant (P<0.0001), while four combinations, Anxa1 and Anxa5, Anxa1 and Vdr, Anxa5 and Vdr, and Lhb and Gnrh-r, were moderately significant (P<0.001). The remaining eight combinations that exhibited statistical significance were Anxa1 and Inha, Anxa1 and Actbb, Vdr and Actbb, Anxa1 and Fshb, Inha and Lhb, Actbb and Fshb, Actbb and Lhb, and Fst and Fshb (P<0.05). These results highlight strong correlations among Anxa1, Anxa5, Vdr, Inha, and Actbb, thereby suggesting that an interaction among ANXA1, ANXA5, and VDR may lead to further communications with inhibin and/or activin in the pituitary gland.
垂体促性腺激素的分泌受多种垂体因子以及 GnRH 和卵巢激素的调节。为了阐明垂体促性腺激素分泌的调节机制，我们观察了发情周期中雌性大鼠垂体中几种垂体因子（即 annexin A1（Anxa1）和 Anxa5、抑制素/激活素亚基、卵泡抑素（Fst）和维生素 D 受体（Vdr））的 mRNA 水平的变化，以及 LHβ亚基（Lhb）、FSHβ亚基（Fshb）和 GnRH 受体（Gnrh-r）mRNA 的水平。在发情前期，Anxa1、Anxa5、Vdr 和抑制素 α 亚基（Inha）的水平最低，而在发情期，激活素 βB 亚基（Actbb）、Lhb 和 Gnrh-r 的水平最低。此外，Fshb 在动情后期的水平最高，而 Fst 没有显著差异。相关分析显示了 16 个具有统计学意义的基因组合。特别是，有四个组合，即 Anxa5 和 Inha、Anxa5 和 Actbb、Inha 和 Vdr 以及 Inha 和 Actbb，具有高度显著性（P<0.0001），而有四个组合，Anxa1 和 Anxa5、Anxa1 和 Vdr、Anxa5 和 Vdr 以及 Lhb 和 Gnrh-r，具有中度显著性（P<0.001）。其余八个具有统计学意义的组合是 Anxa1 和 Inha、Anxa1 和 Actbb、Vdr 和 Actbb、Anxa1 和 Fshb、Inha 和 Lhb、Actbb 和 Fshb、Actbb 和 Lhb 和 Fst 和 Fshb（P<0.05）。这些结果突出了 Anxa1、Anxa5、Vdr、Inha 和 Actbb 之间的强相关性，从而表明 ANXA1、ANXA5 和 VDR 之间的相互作用可能导致与垂体中的抑制素和/或激活素进一步通讯。
