Division of ENT Diseases, Department of Clinical Science, Intervention and Technology, Karolinska Institutet, Stockholm, Sweden.
Department of ENT Diseases, Karolinska University Hospital, Stockholm, Sweden.
Sci Rep. 2022 Jun 15;12(1):9915. doi: 10.1038/s41598-022-13986-5.
Despite the introduction of vaccines, COVID-19 still affects millions of people worldwide. A better understanding of pathophysiology and the discovery of novel therapies are needed. One of the cells of interest in COVID-19 is the neutrophil. This cell type is being recruited to a site of inflammation as one of the first immune cells. In this project, we investigated a variety of neutrophils phenotypes during COVID-19 by measuring the expression of markers for migration, maturity, activation, gelatinase granules and secondary granules using flow cytometry. We show that neutrophils during COVID-19 exhibit altered phenotypes compared to healthy individuals. The activation level including NETs production and maturity of neutrophils seem to last longer during COVID-19 than expected for innate immunity. Neutrophils as one of the drivers of severe cases of COVID-19 are considered as potential treatment targets. However, for a successful implementation of treatment, there is a need for a better understanding of neutrophil functions and phenotypes in COVID-19. Our study answers some of those questions.
尽管已经推出了疫苗，但 COVID-19 仍在全球范围内影响着数百万人。我们需要更好地了解病理生理学并发现新的治疗方法。COVID-19 中感兴趣的细胞之一是中性粒细胞。这种细胞类型作为最早的免疫细胞之一被招募到炎症部位。在这个项目中，我们通过使用流式细胞术测量迁移、成熟、激活、明胶酶颗粒和次级颗粒的标志物，来研究 COVID-19 期间的各种中性粒细胞表型。我们发现，与健康个体相比，COVID-19 期间的中性粒细胞表现出改变的表型。中性粒细胞的激活水平包括 NETs 的产生和成熟，在 COVID-19 期间似乎比预期的先天免疫持续时间更长。中性粒细胞被认为是 COVID-19 严重病例的驱动因素之一，被视为潜在的治疗靶点。然而，为了成功实施治疗，需要更好地了解 COVID-19 中的中性粒细胞功能和表型。我们的研究回答了其中的一些问题。
