Key Laboratory of Precision Nutrition and Food Quality, Key Laboratory of Functional Dairy, Ministry of Education, College of Food Science and Nutritional Engineering, China Agricultural University, 100083, Beijing, China.
Department of Food Quality and Safety, College of Food Science and Engineering, Jilin University, 130062, Changchun, China.
Plant Foods Hum Nutr. 2022 Sep;77(3):367-372. doi: 10.1007/s11130-022-00982-3. Epub 2022 Jun 15.
Gynostemma pentaphyllum (G. pentaphyllum) is a perennial liana herb of the Cucurbitaceae family which has both nutraceutical and pharmacological functions. The objective of the current study was to investigate the preventative effects of G. pentaphyllum and Gypenoside-IV (GP-IV, a saponin monomer in G. pentaphyllum) on metabolic symptoms in high fat diet induced obese (DIO) mice with gut microbiota dysbiosis. G. pentaphyllum water extract (GPWE, 150 mg/kg•d) and GP-IV (50 mg/kg•d) were orally administered to DIO mice by gavage for 10 weeks. The results showed that both GPWE and GP-IV prevented obesity development by decreasing body weight gain, reducing fat mass/body weight ratio and inhibiting adipocyte hypertrophy. GPWE and GP-IV also improved lipid profile and glucose tolerance effectively. Moreover, GPWE and GP-IV treatments partly restored gut microbiota in DIO mice. Typically, GPWE and GP-IV reduced Firmicutes to Bacteroidetes ratio, increased the abundance of certain health-promoting bacteria and reduced the abundance of microbiota that were associated with metabolic disorders. We conclude that GPWE and GP-IV can ameliorate metabolic symptoms possibly via modulating gut microbiota in DIO mice.
绞股蓝（G. pentaphyllum）是葫芦科的一种多年生藤本植物，具有营养和药理作用。本研究旨在探讨绞股蓝水提物（GPWE，150mg/kg•d）和绞股蓝总皂苷单体（GP-IV，50mg/kg•d）对高脂饮食诱导的肥胖（DIO）伴有肠道菌群失调小鼠代谢症状的预防作用。通过灌胃给药，10 周后，DIO 小鼠给予绞股蓝水提物（GPWE，150mg/kg•d）和绞股蓝总皂苷单体（GP-IV，50mg/kg•d）。结果表明，GPWE 和 GP-IV 通过降低体重增加、减少脂肪量/体重比和抑制脂肪细胞肥大来预防肥胖的发展。GPWE 和 GP-IV 还能有效改善血脂谱和葡萄糖耐量。此外，GPWE 和 GP-IV 治疗部分恢复了 DIO 小鼠的肠道微生物群。通常，GPWE 和 GP-IV 降低了厚壁菌门/拟杆菌门的比例，增加了某些有益健康的细菌的丰度，减少了与代谢紊乱相关的微生物的丰度。我们得出结论，GPWE 和 GP-IV 可能通过调节 DIO 小鼠的肠道微生物群来改善代谢症状。
