Center for Excellence in Post-Harvest Technologies, North Carolina Agricultural and Technical State University, North Carolina Research Campus, 500 Laureate Way, Kannapolis, North Carolina 28081, United States.
J Agric Food Chem. 2015 May 20;63(19):4843-52. doi: 10.1021/acs.jafc.5b01246. Epub 2015 May 8.
In this study, we investigated the preventive effects of carnosic acid (CA) as a major bioactive component in rosemary extract (RE) on high-fat-diet-induced obesity and metabolic syndrome in mice. The mice were given a low-fat diet, a high-fat diet or a high-fat diet supplemented with either 0.14% or 0.28% (w/w) CA-enriched RE (containing 80% CA, RE#1L and RE#1H), or 0.5% (w/w) RE (containing 45% CA, RE#2), for a period of 16 weeks. There was the same CA content in the RE#1H and RE#2 diets and half of this amount in the RE#1L diet. The dietary RE supplementation significantly reduced body weight gain, percent of fat, plasma ALT, AST, glucose, insulin levels, liver weight, liver triglyceride, and free fatty acid levels in comparison with the mice fed with a HF diet without RE treatment. RE administration also decreased the levels of plasma and liver malondialdehyde, advanced glycation end products (AGEs), and the liver expression of receptor for AGE (RAGE) in comparison with those for mice of the HF group. Histological analyses of liver samples showed decreased lipid accumulation in hepatocytes in mice administrated with RE in comparison with that of HF-diet-fed mice. Meanwhile, RE administration enhanced fecal lipid excretion to inhibit lipid absorption and increased the liver GSH/GSSG ratio to perform antioxidant activity compared with HF group. Our results demonstrate that rosemary is a promising dietary agent to reduce the risk of obesity and metabolic syndrome.
在这项研究中，我们研究了迷迭香提取物（RE）中的主要生物活性成分鼠尾草酸（CA）对高脂肪饮食诱导的肥胖和代谢综合征的预防作用。将小鼠给予低脂饮食、高脂饮食或高脂饮食补充 0.14%或 0.28%（w/w）富含 CA 的 RE（含有 80%CA、RE#1L 和 RE#1H）或 0.5%（w/w）RE（含有 45%CA、RE#2），为期 16 周。RE#1H 和 RE#2 饮食中的 CA 含量相同，而 RE#1L 饮食中的 CA 含量为其一半。与未用 RE 治疗的 HF 饮食组相比，膳食 RE 补充可显著降低体重增加、脂肪百分比、血浆 ALT、AST、葡萄糖、胰岛素水平、肝重、肝甘油三酯和游离脂肪酸水平。与 HF 组相比，RE 给药还降低了血浆和肝丙二醛、晚期糖基化终产物（AGEs）和肝 AGE 受体（RAGE）的表达水平。与 HF 饮食喂养的小鼠相比，给予 RE 的小鼠肝组织样本中的肝细胞脂质积累减少。同时，与 HF 组相比，RE 给药可增加粪便脂质排泄以抑制脂质吸收，并增加肝脏 GSH/GSSG 比值以发挥抗氧化活性。我们的研究结果表明，迷迭香是一种很有前途的膳食剂，可降低肥胖和代谢综合征的风险。
