大象生长激素。分离与特性鉴定。

Elephant growth hormone. Isolation and characterization.

作者信息

Li C H, Bewley T A, Chung D, Oosthuizen M M

出版信息

Int J Pept Protein Res. 1987 Jan;29(1):62-7. doi: 10.1111/j.1399-3011.1987.tb02230.x.

DOI:10.1111/j.1399-3011.1987.tb02230.x

Abstract

Growth hormone has been purified to homogeneity from elephant pituitary glands. It has 191 amino acids with two disulfide bridges and a single tryptophan residue. The somatotropin activity is only 15% when compared with the bovine hormone in the radioreceptor binding assay. From circular dichroism spectra alpha-helical content of elephant growth hormone is estimated to be 50%. Difference absorption spectra of the hormone suggest the presence of a hydrogen bond between the single Trp and a carboxylate ion.

摘要

生长激素已从大象垂体中纯化至同质。它有191个氨基酸，带有两个二硫键和一个色氨酸残基。在放射受体结合试验中，与牛生长激素相比，其促生长活性仅为15%。从圆二色光谱估计，大象生长激素的α-螺旋含量为50%。该激素的差示吸收光谱表明，单个色氨酸与一个羧酸根离子之间存在氢键。