Yang Pan, Li Wei, Li Xiaoping
Department of Thoracic Surgery, Tianjin First Central Hospital, School of Medicine, Nankai University, Tianjin 300192, China.
J Oncol. 2022 Jun 6;2022:3599832. doi: 10.1155/2022/3599832. eCollection 2022.
The study aims to explore the biological function of SHC1 in the development and progression of lung cancer. Meanwhile, the effect of SHC1 and EGFR on lung cancer was analyzed. The expression of SHC1 in lung cancer and adjacent tissues was analyzed by bioinformatics and immunohistochemistry. Meanwhile, the relationship between SHC1 expression and prognosis was analyzed. SHC1 overexpression and knockdown cell lines were constructed by overexpression plasmid and knockdown plasmid. Cell proliferation was detected by CCK-8. Cell invasion was detected by transwell. Apoptosis was detected by TUNEL. Interaction between SHC1 and EGFR was detected. The expression of SHC1 in lung adenocarcinoma tissues was significantly higher than that in paracancer tissues. Lung cancer patients with high SHC1 expression have a poor prognosis. The proliferation and invasion of SHC1 decreased with SHC1 knockout but increased after overexpression. EGFR may be a key interaction protein of SHC1. Overexpression of EGFR increases the oncogenic effect of SHC1. In conclusion, SHC1 plays a carcinogenic role in lung cancer. EGFR expression was significantly correlated with SHC1 and maybe a key interaction protein of SHC1. SHC1 interacts with EGFR to form a protein complex, which may be a new target for lung cancer metastasis.
本研究旨在探讨SHC1在肺癌发生发展过程中的生物学功能。同时，分析SHC1和表皮生长因子受体（EGFR）对肺癌的影响。通过生物信息学和免疫组织化学分析SHC1在肺癌组织和癌旁组织中的表达情况。同时，分析SHC1表达与预后的关系。利用过表达质粒和敲低质粒构建SHC1过表达和敲低细胞系。采用CCK-8法检测细胞增殖情况。通过Transwell法检测细胞侵袭能力。采用TUNEL法检测细胞凋亡情况。检测SHC1与EGFR之间的相互作用。SHC1在肺腺癌组织中的表达明显高于癌旁组织。SHC1高表达的肺癌患者预后较差。敲除SHC1后其增殖和侵袭能力降低，而过表达后则增强。EGFR可能是SHC1的关键相互作用蛋白。EGFR的过表达增强了SHC1的致癌作用。综上所述，SHC1在肺癌中发挥致癌作用。EGFR表达与SHC1显著相关，可能是SHC1的关键相互作用蛋白。SHC1与EGFR相互作用形成蛋白复合物，这可能是肺癌转移的新靶点。
