Scientific Research Center, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, Guangdong 518107, China.
J Immunol Res. 2022 Jun 6;2022:7623654. doi: 10.1155/2022/7623654. eCollection 2022.
Lectin, Mannose Binding 2 (LMAN2) encodes a type I transmembrane lectin that shuttles between the plasma membrane, the Golgi apparatus, and the endoplasmic reticulum. However, its expression, prognosis, and function in invasive breast carcinoma remain unknown. Nine databases were consulted to evaluate LMAN2 expression and prognosis in breast cancer. The possible function of LMAN2 in breast cancer was investigated in the Human Cell Landscape (HCL) database, Gene Regulatory Network database (GRNdb), and CancerSEA database. Moreover, N6-methyladenosine (m6A) modifications were analyzed using the RMBase v2.0 and M6A2Target databases. Seven databases were then used to analyze the potential action mechanisms of LMAN2. Our findings suggest that LMAN2, which is expressed at a high level in breast cancer, is linked to an unfavorable prognosis. Therefore, LMAN2 has the potential to be utilized as a treatment target in breast cancer. Furthermore, the single-cell analysis illustrated that LMAN2 expression had a positive link to breast cancer stemness, proliferation, metastasis, and differentiation. Moreover, m6A modifications were found in the LMAN2 gene. Consequently, modifications to m6A methylation may influence LMAN2 expression, which is associated with the homologous recombination (HR) in its DNA damage repair pathway .
凝集素，甘露糖结合 2（LMAN2）编码一种Ⅰ型跨膜凝集素，在质膜、高尔基体和内质网之间穿梭。然而，其在浸润性乳腺癌中的表达、预后和功能仍不清楚。本研究检索了 9 个数据库来评估 LMAN2 在乳腺癌中的表达和预后。在 Human Cell Landscape（HCL）数据库、Gene Regulatory Network database（GRNdb）和 CancerSEA 数据库中研究了 LMAN2 在乳腺癌中的可能功能。此外，使用 RMBase v2.0 和 M6A2Target 数据库分析了 N6-甲基腺苷（m6A）修饰。然后，使用 7 个数据库来分析 LMAN2 的潜在作用机制。我们的研究结果表明，在乳腺癌中高表达的 LMAN2 与不良预后相关。因此，LMAN2 有可能被用作乳腺癌的治疗靶点。此外，单细胞分析表明 LMAN2 表达与乳腺癌干性、增殖、转移和分化呈正相关。此外，在 LMAN2 基因中发现了 m6A 修饰。因此，m6A 甲基化修饰可能会影响 LMAN2 的表达，进而影响其 DNA 损伤修复途径中的同源重组（HR）。
