从阿波美-卡拉维公社0至5岁儿童肠道微生物群中分离出的[物质名称]的特征 。（原文中“Isolated from Intestinal Microbiota Children of 0-5 Years Old”部分表述不太准确规范，推测可能是某种物质从儿童肠道微生物群中被分离出来，但缺少具体所指物质，这里按推测补充了[物质名称]进行翻译）
Characteristics of Isolated from Intestinal Microbiota Children of 0-5 Years Old in the Commune of Abomey-Calavi.
作者信息
Sina Haziz, Dah-Nouvlessounon Durand, Adjobimey Tomabu, Boya Bawa, Dohoue Ghislaine M C, N'tcha Christine, Chidikofan Violette, Baba-Moussa Farid, Abdoulaye Idrissou, Adjanohoun Adolphe, Baba-Moussa Lamine
机构信息
Laboratory of Biology and Molecular Typing in Microbiology, Department of Biochemistry and Cellular Biology, Faculty of Sciences and Techniques, University of Abomey-Calavi, 05 BP 1604 Cotonou, Godomey, Benin.
Biochemistry and Molecular Biology Unit, Department of Biochemistry and Cellular Biology, Faculty of Sciences and Techniques, University of Abomey-Calavi, Godomey, Benin.
出版信息
J Pathog. 2022 Jun 6;2022:6253894. doi: 10.1155/2022/6253894. eCollection 2022.
is a commensal bacterium and one of the first bacteria to colonize the digestive tract of newborns after birth. It is characterized by great versatility and metabolic flexibility that allows its survival in different niches. The present study aims at analyzing the diversity of strains isolated from the intestinal microbiota of children aged from 0 to 5 years in the commune of Abomey-Calavi in Benin. For this purpose, a descriptive and analytical cross-sectional study was conducted. A total of 135 stool samples were collected from the pediatric clinic of Abomey-Calavi. Microbiological analyses were performed according to standard microbiology analytical techniques. The molecular characterization of was performed by investigating eight genes (dinB, icdA, pabB, polB, putP, trpA, trpB, and uidA) using the PCR technique. The results showed that the average loading rate on stool samples was 3.74 × 10 CFU/g for TAMF. A total of 7 species of bacteria were identified at different proportions: (55.36%), (14.29%), (12.5%), (5.36%), and (5.36%). Interestingly, isolated presented a resistance of 100% to cefotaxime and aztreonam. In addition, resistances of 95.24% and 50% were observed against erythromycin and nalidixic acid, respectively. The molecular characterization of the isolated strains allowed us to discover another molecular variation within the isolated strains. Genes encoding the enzymes isocitrate dehydrogenase (icd) and DNA polymerase II (polB) were detected at 96.30% in the isolated strains. Moreover, the genes encoding the enzymes beta-D-glucuronidase (A) and DNA polymerase (dinB) were detected at 88.89% in the isolated strains. Interestingly, 81.48%, 85.19, 92.59%, and 100% of isolated strains expressed the genes encoding the enzymes tryptophan synthase subunit A (trpA), proline permease (P), p-aminobenzoate synthase, and tryptophan synthase subunit B (trpB), respectively. The diversity of strains reflects the importance of regulatory mechanisms in the adaptation of bacteria to the gut microbiota.
是一种共生菌，也是新生儿出生后最早定殖于消化道的细菌之一。其特点是具有很强的通用性和代谢灵活性，使其能够在不同生态位中生存。本研究旨在分析从贝宁阿波美 - 卡拉维市0至5岁儿童肠道微生物群中分离出的菌株的多样性。为此，进行了一项描述性和分析性横断面研究。从阿波美 - 卡拉维市的儿科诊所共收集了135份粪便样本。微生物学分析按照标准微生物学分析技术进行。通过使用PCR技术研究八个基因（dinB、icdA、pabB、polB、putP、trpA、trpB和uidA）对进行分子表征。结果表明，粪便样本中TAMF的平均载量率为3.74×10 CFU/g。共鉴定出7种不同比例的细菌：（55.36%）、（14.29%）、（12.5%）、（5.36%）和（5.36%）。有趣的是，分离出的对头孢噻肟和氨曲南的耐药性为100%。此外，观察到对红霉素和萘啶酸的耐药性分别为95.24%和50%。对分离出的菌株进行分子表征使我们能够在分离出的菌株中发现另一种分子变异。在分离出的菌株中，编码异柠檬酸脱氢酶（icd）和DNA聚合酶II（polB）的基因检测率为96.30%。此外，在分离出的菌株中，编码β - D - 葡萄糖醛酸酶（A）和DNA聚合酶（dinB）的基因检测率为88.89%。有趣的是，分离出的菌株中分别有81.48%、85.19%、92.59%和100%表达了编码色氨酸合酶亚基A（trpA）、脯氨酸通透酶（P）、对氨基苯甲酸合酶和色氨酸合酶亚基B（trpB）的基因。菌株的多样性反映了调节机制在细菌适应肠道微生物群中的重要性。
Zotero 插件